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product name
RBL-1, 86061001
biological source
rat blood
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat basophilic leukaemia granulocyte
Cell Line Description
Exhibits marked variations in differentiation when monitored for basophilic granule development, histamine content and membrane receptor sites for IgE.
Application
Differentiation studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10-20% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 5% CO2; 37°C. Treat as suspension when sub-culturing. Some fibroblast-like cells within the population will attach. Please disregard these and subculture as per normal suspension cell lines.
Other Notes
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Certificates of Analysis (COA)
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