We would recommend coupling the ligand immediately after washing and preparing the resin. This is because the reactive groups hydrolyze in the basic solution conditions of the preparation steps. The solutions used in the coupling reaction should also be kept cold (2-8°C) during the procedure.
C9142
Cyanogen bromide-activated-Sepharose™ 4B
lyophilized powder
Synonym(s):
Cyanogen bromide-activated Agarose
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About This Item
Pricing and availability is not currently available.
form
lyophilized powder
Quality Segment
technique(s)
affinity chromatography: suitable
matrix
Sepharose 4B
matrix active group
cyanate or related structures
matrix spacer
1 atom (when ligands are coupled via isourea derivative or related linkage)
swelling
1 g swells to 4-5 mL
particle size
45—165 μm
storage temp.
2-8°C
General description
Cyanogen bromide-activated-Sepharose™ is suitable for use in affinity chromatography and protein chromatography. It has been used to discover insecticidal agents, raise polyclonal antibodies against yeast invertase glycosyls, and study immunological processes of xenograft rejection.
C9142-15G′s updated product number is GE17-0430-01
C9142-15G′s updated product number is GE17-0430-01
Application
Cyanogen bromide-activated-Sepharose™ 4B has been used:
- in the purification of clusterin from serum by an immunoaffinity-based separation procedure
- in affinity chromatography to isolate Leishmania donovani protein antigens from the urine of visceral leishmaniasis patients
- in affinity chromatography for the isolation of haptoglobin from human serum
Physical form
Lyophilized powder stabilized with lactose and dextran
Legal Information
Sepharose is a trademark of Cytiva
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This Item | |||
|---|---|---|---|
| matrix Sepharose 4B | matrix Sepharose 4 Fast Flow | matrix Sepharose 6B | matrix agarose, 4% cross-linked |
| matrix active group cyanate or related structures | matrix active group cyanate or related structures | matrix active group epoxy | matrix active group - |
| particle size 45—165 μm | particle size 45—165 μm | particle size 45—165 μm | particle size 45-165 μm |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form suspension |
| technique(s) affinity chromatography: suitable | technique(s) affinity chromatography: suitable | technique(s) affinity chromatography: suitable | technique(s) - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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After I've washed and prepared the resin in Product C9142, Cyanogen bromide-activated-Sepharose®, for the coupling reaction, how long can I wait before coupling the ligand to the resin?
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My ligand is in Tris buffer. Can I use Product C9142, Cyanogen bromide-activated-Sepharose®, with this sample?
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Because Tris is an amine-containing buffer, we do not recommend coupling of samples in Tris buffer to the CNBr-activated Sepharose®. Tris will react with the CNBr groups and remove sites for ligand coupling. We would not recommend use of any amine-containing buffer in the coupling reaction. However, after the ligand has been added in the coupling reaction, then it is OK to use amine-containing buffers to block the remaining sites (e.g. ethanolamine, glycine).
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When using Product C9142, Cyanogen bromide-activated-Sepharose®, is there a particular storage buffer that I should use after I've coupled my ligand to the resin?
1 answer-
This depends on the particular situation, and the answer depends more on the coupled ligand than the resin itself. The most important criterion is to choose a buffer that is not harmful to the ligand. It is common to include 20% ethanol or preservatives like sodium azide or thimerosal (~0.02%) to inhibit potential bacterial growth.
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How are proteins attached to the Cyanogen bromide-activated Sepharose® in Product C9142?
1 answer-
Coupling of molecules to CNBr-activated Sepharose® is usually via amine groups. In the case of proteins, this usually means the epsilon amines (exocyclic amines) of lysine groups.
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What kind of volume should I use for my ligand to couple to the Cyanogen bromide-activated Sepharose®, Product C9142?
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It is best that the coupling ligand should not be in too dilute a solution. In general, a volume ratio of 0.5 to 1 of coupling solution to CNBr-activated Sepharose® is recommended.
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Should I use a desiccator environment for storage of Cyanogen bromide-activated-Sepharose®?
1 answer-
It is a good idea to store the CNBr-activated agarose products in as dry an environment as possible, because the reactive groups are very moisture-sensitive.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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