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C8058

Chondroitinase B from Flavobacterium heparinum

lyophilized powder (with BSA as stabilizer)

Synonym(s):

Chondroitin sulfate B lyase

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About This Item

CAS Number:
52227-83-5
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
MFCD00166444
Biological source:
bacterial (Flavobacterium heparinum)
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biological source

bacterial (Flavobacterium heparinum)

Quality Segment

conjugate

(Glucosaminoglycan)

form

lyophilized powder (with BSA as stabilizer)

shipped in

dry ice

storage temp.

−20°C

Application

The enzyme from Sigma has been used for the analysis of the frequency of iduronic acid in dermatan sulfate dodecasaccharide. It has also been used to digest dermatan sufate (DS) in melanoma cells. This digestion resulted in decreased proliferation and invasiveness of tumor cells, thereby suggesting a role for DS in metastasis.
Chondroitinase B from Flavobacterium heparinum has been used in a study to assess the structural characterization and antithrombin activity of dermatan sulfate. Chondroitinase B from Flavobacterium heparinum has also been used in a study to investigate the chondroitin lyase action pattern via liquid chromatography–mass spectrometry.

Biochem/physiol Actions

Chondroitinase B degrades only chondroitin sulphate B producing oligo- and tetra-saccharides, and an unsaturated 4-sulphated disaccharide. It has an optimum temperature of 20 °C and an optimum pH of 8.0. The enzyme activity is inhibited by 50% using 0.1 M NaCI. Co2+, Fe3+ and Ba2+ also inhibit its activity.

Other Notes

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25°C using chondroitin sulfate B as substrate.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Codes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

13 - Non Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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