C2276

Z-Gly-Pro-Arg p-nitroanilide acetate salt

protease substrate

• Empirical Formula (Hill Notation): C₂₇H₃₄N₈O₇ · xC₂H₄O₂
• CAS Number: 66648-35-9
• Molecular Weight: 582.61 (free base basis)
• NACRES: NA.32
• PubChem Substance ID: 329774987
• UNSPSC Code: 12352204
• MDL number: MFCD18782526

Properties

• Quality Segment: 200
• assay: ≥98% (TLC)
• form: powder
• solubility: methanol: 20 mg/mL, clear, colorless to light yellow
• storage temp.: −20°C
• SMILES string: CC(O)=O.NC(=N)NCCC[C@H](NC(=O)[C@@H]1CCCN1C(=O)CNC(=O)OCc2ccccc2)C(=O)Nc3ccc(cc3)[N+]([O-])=O
• InChI: 1S/C27H34N8O7.C2H4O2/c28-26(29)30-14-4-8-21(24(37)32-19-10-12-20(13-11-19)35(40)41)33-25(38)22-9-5-15-34(22)23(36)16-31-27(39)42-17-18-6-2-1-3-7-18;1-2(3)4/h1-3,6-7,10-13,21-22H,4-5,8-9,14-17H2,(H,31,39)(H,32,37)(H,33,38)(H4,28,29,30);1H3,(H,3,4)/t21-,22-;/m0./s1
• InChI key: FPMAXHHYPVIJMC-VROPFNGYSA-N

Description

Application

Z-Gly-Pro-Arg p-nitroanilide acetate salt has been used as a substrate in prostate-specific antigen (PSA) activation protease assay for determining enterokinase-PSA-trypsin (EK-PSA-TRP) activity and to perform kinetic experiments. It has also been used as a substrate to measure the pancreatic trypsin activity chromogenically.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)