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Protein Extraction Reagent Type 4

Name: Protein Extraction Reagent Type 4
Brand: SIGMA

Synonym(s):

Protein extraction solution

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About This Item

NACRES:

NA.56

UNSPSC Code:

41116012

Pricing and availability is not currently available.

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Properties

form

solid

Quality Segment

200

input

general use sample(s)

solubility

deionized water: 13.6 g /15 ml at 25 °C, clear to hazy

Description

General description

Protein Extraction Reagent Type 4, is a highly premixed, prequalified and effective protein solubilization and extraction reagent composed of urea, thiourea, Trizma^®, and detergent C7BzO (C0856).

Application

Protein extraction reagent type 4 is optimized for proteomic studies. Cellular and organelle membrane solubilizing reagent is ideal for protein extraction prior to isoelectric focusing and 2D electrophoresis. Reducing and alkylating reagents TBP (T7567) and iodoacetamide (A3221) are recommended for use with this reagent. Protein extraction reagent type 4 is also available as a component of the ProteoPrep^® Sample.

Other Notes

Contains urea, thiourea, Trizma^®, and detergent C7BzO (C0856).

Legal Information

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item	70922	71296	71186
Sigma-Aldrich C0356 Protein Extraction Reagent Type 4	Millipore 70922 BugBuster^® HT Protein Extraction Reagent	Millipore 71296 PhosphoSafe Extraction Reagent	Millipore 71186 YeastBuster Protein Extraction Reagent
description -	description -	description Novagen′s PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state.	description Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis
form solid	form liquid	form liquid	form liquid
solubility deionized water: 13.6 g /15 ml at 25 °C, clear to hazy	solubility -	solubility -	solubility -
input general use sample(s)	input bacteria and yeast sample(s)	input general use sample(s)	input bacteria and yeast sample(s)
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level -

pictograms

GHS08,GHS07,GHS09

signalword

Warning

hcodes

H302,H351,H361fd,H411

pcodes

P202 - P264 - P273 - P280 - P301 + P312 - P308 + P313

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Repr. 2

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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