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B6429

Casein Blocking Buffer 10x

for Northern and Southern blotting, solution

Synonym(s):

10x Blocking Buffer

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About This Item

NACRES:
NA.54
UNSPSC Code:
12161700
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grade

Molecular Biology

Quality Level

200

form

solution

technique(s)

Southern blotting: suitable

storage temp.

room temp

General description

Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.[1]
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents[1] to the Northern and Southern Blot membranes.

Application

Casein Blocking Buffer 10x has been used in:

  • western blotting[2][3][4]
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemistry

Features and Benefits

  • Better blocking performance than dried milk or BSA
  • Easy-to-use concentrate
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent and colorimetric detection assays

Preparation Note

Dilute 10x Blocking Buffer with molecular biology grade water before use.

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This Item
C7594G766393017
Casein Blocking Buffer 10x for Northern and Southern blotting, solution

Sigma-Aldrich

B6429

Casein Blocking Buffer 10x

Casein Blocking Buffer for Northern and Southern blotting, powder blend

Sigma-Aldrich

C7594

Casein Blocking Buffer

Gelatin blocking buffer for Western blotting, powder blend

Sigma-Aldrich

G7663

Gelatin blocking buffer

SSC Buffer 20x for Northern and Southern blotting, solution

Sigma-Aldrich

93017

SSC Buffer 20x

technique(s)

Southern blotting: suitable

technique(s)

Southern blotting: suitable

technique(s)

-

technique(s)

Southern blotting: suitable

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

form

solution

form

powder blend

form

powder blend

form

solution

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000531163
0000531163
0000500147
0000500147
0000484834

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Global Trade Item Number

SKUGTIN
B6429-500ML04061833434796

Questions

1–8 of 8 Questions  

  1. Is Casein Blocking Buffer (B6429) compatible with SSC-based pre-hybridization or hybridization buffers for Northern blotting (e.g. 6× SSC, 0.5% SDS) with directly fluorescently labeled probes, or is it intended only for post-hybridization blocking steps?

    1 answer

    1. The 10X Casein Blocking Buffer is recommended for both colorimetric and chemiluminescent detection. It is used as a blocking solution for detection, which occurs after hybridization and is suitable for Northern and Southern applications.

      Please also review our Technical Document, Northern & Southern Blot Protocols, available at the link below:
      https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/gel-electrophoresis/southern-and-northern-blotting

      Helpful?

  2. Hi, how much time can be the casein blocking buffer 10X stored from its opening?

    1 answer

    1. This product does not have an expiration date once opened. The datasheet indicates the following regarding storage and stability:

      Storage/Stability: Store the 10X Casein Blocking Buffer at room temperature. It is also stable when stored at 2–8°C. Casein may precipitate over time, which is normal and should not impact the performance of the 10X Blocking Buffer. If precipitation is observed, resuspension of the solids can be achieved by inverting the bottle several times or by vortexing for a few seconds.

      Helpful?

  3. Hi, would it be possible to know the % of casein in your Casein Blocking Buffer 10X Cat# B6429? Thank you.

    1 answer

    1. The composition of this product is proprietary and cannot be disclosed. However, it can be stated that the product is a blocking reagent derived from bovine milk casein and does not contain any serum albumin.

      Helpful?

  4. whats the percentage casein in 10X of B6429-500ml ?

    1 answer

    1. Unfortunately, the concentration of casein is considered proprietary.

      Helpful?

  5. Will my RNA samples and probes be safe with this buffer if we are using horseradish peroxidase and chemiluminescent detection?

    1 answer

    1. The 10X Casein Blocking Buffer is recommended for both colorimetric and chemiluminescent detection. It is used as a blocking solution for detection, which occurs after hybridization and is suitable for Northern and Southern applications.

      Helpful?

  6. What is the pH? What is the counter ion? Is there saline salt in the preparation (0.15M)?

    1 answer

    1. Unfortunately, the composition of this product is proprietary and can't be disclosed.

      Helpful?

  7. What is the % w/v casein in this solution?

    1 answer

    1. The casein concentration is 1.8% of the total solution.

      Helpful?

  8. What is the solution solvent? What grade of reagent is this in terms of sterility and associated testing?

    1 answer

    1. As mentioned in the product detail page, this product is a molecular biology-grade product. A solution is prepared by diluting the 10x Blocking Buffer with molecular biology-grade water.

      Helpful?

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