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form
liquid
packaging
pkg of 250 mL (AC-60250 (HighPrep™ PCR (250 mL)))
pkg of 5 mL (AC-60005 (HighPrep™ PCR (5 mL)))
pkg of 50 mL (AC-60050 (HighPrep™ PCR (50 mL)))
pkg of 500 mL (AC-60500 (HighPrep™ PCR (500 mL)))
storage condition
do not freeze
storage temp.
2-8°C
General description
Magnetic beads-based reagent for manual and for automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and amplicon size selection.
The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.
The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.
Application
HighPrep™ PCR Clean-up System may be used in:
- Post-PCR and post-enzymatic reaction clean-up
- NGS library preparation
- Amplicon size selection for NGS
- Microarrays
- PCR
- Sanger sequencing
- Restriction enzyme digestions, adapter ligations
- Cloning
Features and Benefits
- High recovery of >100 bp amplicons
- Adaptable to high throughput liquid handling systems
- Stable recovery of amplicons greater than 100bp, predictable and consistent size selection
- No centrifugation or filtration leads to higher and purer yields
Storage and Stability
14 months when stored as directed
Legal Information
HiPrep is a trademark of Cytiva
HighPrep is a trademark of MagBio Genomics, Inc.
Disclaimer
For Research Use Only. Not for Use in Diagnostic Procedures.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Vaishali Sah et al.
Gene, 731, 144356-144356 (2020-01-15)
The genetic basis of differential host immune response vis-à-vis transcriptome profile was explored in PBMCs of indigenous (Ghurrah) and crossbred pigs after classical swine fever vaccination and in monocyte derived macrophages (MDMs) challenged with virulent classical swine fever (CSF) virus.
Deep sequencing of near full-length HIV-1 genomes from plasma identifies circulating subtype C and infrequent occurrence of AC recombinant form in Southern India}
Alampalli SV, et al.
PLoS ONE, 17 (2017)
Signature of genome wide gene expression in classical swine fever virus infected macrophages and PBMCs of indigenous vis-a-vis crossbred pigs
Sah V, et al
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