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Dulbecco′s Modified Eagle′s Medium/High Modified

Name: Dulbecco′s Modified Eagle′s Medium/High Modified
Brand: SIGMA

with 4500 mg/L dextrose, with 110mg/L sodium pyruvate, without L-glutamine, without phenol red, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Dulbecco’s Modified Eagle’s Medium - high glucose, DME, DMEM

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

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Properties

description

for research or for further manufacturing use

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable, cell culture | stem cell: suitable

components

phenol red: no
glucose: high
sodium pyruvate: yes
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

Description

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additonally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High". Dulbecco′s Modified Eagle′s Medium/High Modified has been used as a supplement in cell culture.

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

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This Item	51441C	56499C	D0422
SAFC 51449C Dulbecco′s Modified Eagle′s Medium/High Modified	Sigma-Aldrich 51441C Dulbecco′s Modified Eagle′s Medium/High Modified	SAFC 56499C Dulbecco′s Modified Eagle′s Medium/High Modified	Sigma-Aldrich D0422 Dulbecco′s Modified Eagle′s Medium - high glucose
description with 4500 mg/L dextrose, with 110mg/L sodium pyruvate, without L-glutamine, without phenol red, liquid, sterile-filtered, suitable for cell culture	description liquid, sterile-filtered, with 4500 mg/L dextrose, with 4.0 mM L-glutamine, without sodium pyruvate, suitable for cell culture	description with 4500 mg/L dextrose, with 4.0 mM L-glutamine, with 110 mg/L sodium pyruvate, without sodium bicarbonate, dry powder, suitable for cell culture DME, DMEM, Dulbecco’s Modified Eagle’s Medium – high Glucose. Use 13.49 g/L See information sheet, 56499C @ www.sigmaaldrich.com for hydration instructions.	description With 4500 mg/L glucose and sodium bicarbonate, without L-methionine, L-cystine and L-glutamine, liquid, sterile-filtered, suitable for cell culture
sterility sterile-filtered	sterility sterile-filtered	sterility -	sterility sterile-filtered
technique(s) cell culture \| stem cell: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form liquid	form liquid	form powder	form liquid
components phenol red: no glucose: high sodium pyruvate: yes L-glutamine: no	components glucose: high phenol red: yes sodium pyruvate: no L-glutamine: yes	components phenol red: yes sodium pyruvate: yes L-glutamine: yes glucose: high	components glucose: high phenol red: yes L-glutamine: no sodium pyruvate: yes
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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