157M-9

CD57 (NK-1) Mouse Monoclonal Antibody

• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: NK-1, monoclonal
• Application: IHC (p)
• Citations: 5

Properties

• biological source: mouse
• Quality Segment: 100, 500
• conjugate: unconjugated
• antibody form: diluted ascites fluid
• antibody product type: primary antibodies
• clone: NK-1, monoclonal
• description: For In Vitro Diagnostic Use in Select Regions (See Chart)
• form: buffered aqueous solution
• species reactivity: human
• packaging: vial of 0.1 mL concentrate (157M-94), vial of 0.5 mL concentrate (157M-95), bottle of 1.0 mL predilute (157M-97), vial of 1.0 mL concentrate (157M-96), bottle of 7.0 mL predilute (157M-98)
• manufacturer/tradename: Cell Marque^®
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
• isotype: IgMκ
• control: tonsil
• shipped in: wet ice
• storage temp.: 2-8°C
• visualization: membranous
• Gene Information: human ... B3GAT1(27087)

Description

General description

CD57, also known as HNK-1 (human natural killer-1), is a cell surface carbohydrate epitope expressed on terminally differentiated T-cells and subsets of natural killer (NK) cells. It has also been identified in cells of neural crest origin. CD57 antibodies are often used to visualize non-neoplastic bystander T-cells. These T-cells have been known to form rosettes around neoplastic lymphocyte-predominant (LP) cells in nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL).

CD57 is a marker expressed in the membrane of NK cells and other T cells. It is often used in the identification of nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) to visualize the small T-cells that ring the LP cells (rosettes of follicular T cells around the large neoplastic LP cells). These features can be used to distinguish NLPHL from T-cell/histiocyte-rich large B-cell lymphoma, which usually shows no rosettes of CD57-positive follicular T-cells around the large tumor cells. Anti-CD57 also reacts with neuroendocrine cells and their derived tumors, including neuroendocrine tumors of diverse origins, pheochromocytomas, paragangliomas, medulloblastoma, and varying proportions of neural tumors such as schwannomas, neurofibromas, neuromas, and granular cell tumors. However, anti-CD57 lacks the specificity of chromogranin and synaptophysin for detecting neuroendocrine neoplasms and therefore should be considered in a panel. Anti-CD57 can also be useful in separating thymoma from thymic carcinoma when combined with a panel that includes antibodies against CD5 and CD117.

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Analysis Note

IVD

IVD

IVD

RUO

Other Notes

CD57 Positive Control Slides, Product No. 157S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

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Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany