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39727

Millipore

Perfringens Agar Base

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSC Code:
41171609
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

composition

Agar, 15 g/L
Beef extract, 5 g/L
Ferric ammonium citrate, 1 g/L
Papaic digest of soybean meal, 5 g/L
Sodium metabisulphite, 1 g/L
Tryptose, 15 g/L
Yeast extract, 5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

application(s)

bioburden testing
cleaning products
cosmetics
environmental
food and beverages
personal care

microbiology

suitability

selective and differential for Clostridium spp.

General description

Perfringens Agar Base with the addition of selective supplement and enrichment is used for the presumptive identification and enumeration of Clostridium perfringens from food.

Application

Perfringens Agar Base has been used in the cell culture of Clostridium perfringens.

Preparation Note

Suspend 23.5 g of Perfringens Agar Base in 475 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C. Add 25 ml of Egg Yolk Emulsion (Cat. No. 17148) and additionally the rehydrated contents of 1 vial of Perfringens S.F.P. Supplement (Cat. No. 53436) or 1 vial of Perfringens T.S.C. Supplement (Cat. No. P9352). Mix well and pour into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Martina Hustá et al.
Anaerobe, 63, 102205-102205 (2020-05-01)
Isolation and fast detection of Clostridium perfringens is essential in veterinary medical diagnostics and veterinary research, as it allows to recommend suitable treatment options after antimicrobial resistance determination, and is essential to study pathogenesis. In this study four selective media

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