198161

Toluidine Blue O

certified by the Biological Stain Commission

• Synonym(s): Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride
• Empirical Formula (Hill Notation): C₁₅H₁₆ClN₃S
• CAS Number: 92-31-9
• Molecular Weight: 305.83
• Colour Index Number: 52040
• EC Number: 202-146-2
• MDL number: MFCD00011934
• UNSPSC Code: 12171500
• PubChem Substance ID: 24851865
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• composition: Dye content, 80%
• color: dark green
• λmax: 626 nm
• ε (extinction coefficient): ≥13000 at 241-247 nm in H₂O; ≥28000 at 287-293 nm in H₂O; ≥40000 at 625-635 nm in H₂O
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C
• InChI: 1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H
• InChI key: GEDVVYWLPUPJJZ-UHFFFAOYSA-N

Description

General description

A metachromatic dye suitable for a wide variety of histological staining procedures.

Application

Toluidine Blue O along with light-emitting diode has been used as a photosensitizer to control in vitro biofilms and to kill gram-negative bacteria.

Biochem/physiol Actions

Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite. TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries. This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin.

Suitability

Certified for use as a metachromatic stain for mast cells and cartilage using the Churukian-Schenk method and the method of Nocht on paraffin sections.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves