05121

Heart Infusion Broth

NutriSelect^® Plus, suitable for microbiology

• Synonym(s): HIB
• UNSPSC Code: 41171607
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: beef heart (infusion from 500 g), 10 g/L ; sodium chloride, 5.0 g/L ; tryptose, 10.0 g/L
• packaging: bottle of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• color: light yellow
• final pH: 7.4±0.2 (25 °C)
• application(s): clinical testing; environmental; food and beverages; microbiology
• suitability: nonselective for Escherichia coli; nonselective for Neisseria spp.; nonselective for Staphylococcus spp.; nonselective for Streptococcus spp.; nonselective for bacteria (General Media); nonselective for coliforms

Description

General description

Heart Infusion Broth, containing infusion from the beef heart, is used for the isolation and cultivation of a wide variety of fastidious organisms.The fastidious organisms having challenging nutritional requirements could be cultivated on infusion media. A liquid medium containing an infusion of meat was one of the first media used for the cultivation of bacteria. The heart infusion media requires no further supplements to cultivate fastidious bacteria. The media contains Tryptose and beef heart infusions, which provide nitrogenous compounds, amino acids, and other essential growth nutrients required for the growth pathogenic bacteria. Sodium chloride maintains the osmotic equilibrium of the medium. Heart Infusion Broth is also recommended for Vibrio cholerae and Vibrio species and can be used as the base in carbohydrate fermentation tests. This medium is also used for the isolation and enumeration of hemolytic Streptococci in milk.

Application

Heart Infusion Broth is used for general laboratory purposes for the cultivation of fastidious organisms from various test samples.

Features and Benefits

NutriSelect^® Plus are powdered culture media offering

• Quality control with growth promotion test according to industry standards
• Quality assurance acc. to ISO 9001
• Cost-efficient powdered media

Preparation Note

Suspend 25 g in 1000 ml of purified water. Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. If desired 5% sterile defibrinated blood may be added. Mix well and pour sterile petri plates or tubes.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves