SCM103
EmbryoMax® MEF Conditioned Media
EmbryoMax MEF Conditioned Media (MEF-CM) can be used for the feeder-free culture of human ES/iPS cells.
Synonym(s):
MEFCM, MEF-CM, Mouse Embryonic Fibroblast Conditioned Media
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.71
Quality Level
manufacturer/tradename
Specialty Media
EmbryoMax®
technique(s)
cell culture | stem cell: suitable
shipped in
ambient
General description
Traditionally, human embryonic stem cell (hESC) or induced pluripotent stem cell (iPSC) culture require mouse embryonic fibroblasts (MEF) support. This requirement makes the protocol complicated and variable. MEF conditioned medium (MEFCM) contains all the growth factors needed in hESC/iPSC culture, eliminating the routine preparation of feeder cells. It is a ready-to-use product for the feeder-free culture of human ES/IPS cells.
Each batch of EmbryoMax MEF Conditioned Media has been tested for hES/iPS cells pluripotency. The media requires an additional 10 ng/ml of bFGF to be added once thawed before use.
Each batch of EmbryoMax MEF Conditioned Media has been tested for hES/iPS cells pluripotency. The media requires an additional 10 ng/ml of bFGF to be added once thawed before use.
Cell Line Description
Primary Mouse Embryo Fibroblasts
Quality
Mycoplasma Testing: PASSED
Bacterial Testing: PASSED
Fungi Testing: PASSED
Bacterial Testing: PASSED
Fungi Testing: PASSED
Storage and Stability
Store at -80C until expiration date on label.
Legal Information
EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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