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SCM045

Human Mesenchymal-XF Expansion Medium

Xeno-free mesenchymal stem cell media specially formulated for the rapid expansion of human mesenchymal stem cells isolated from a variety of sources, including adipose and bone marrow tissues.

Synonym(s):

Human MSC Culture Media

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
eCl@ss:
32160801
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Quality Segment

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type mesenchymal stem cell(s)

shipped in

dry ice

storage temp.

-10 to -25°C

General description

The Human Mesenchymal-XF Expansion Medium is a xeno-free medium specially formulated for the rapid expansion of human mesenchymal stem cells isolated from a variety of sources, including adipose and bone marrow tissues. The medium contains 2% human serum, is free of animal-derived components and supports the growth of human MSCs at rates equal to or greater than other commercial xeno-free/serum-free media and media supplemented with 2-10% FBS. Human MSCs cultured in the medium retain self-renewal and multi-lineage differenitation potential. The Human Mesenchymal-XF Expansion Medium does not contain any antimicrobials, phenol red, or any other components that can cause cell stress and “masking effects” that may influence experimental results.

Application

A separate ECM solution is not required to facilitate cell attachment using the human mesenchymal-XF expansion medium.
Research Category
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells

Physical form

The Human Mesenchymal-XF Expansion Medium is a complete medium and contains all the necessary growth factors and supplements to support human mesenchymal stem cell growth.

Preparation Note

All components used within the media are of human origin and do not contain any animal derived components.
Frozen medium should be stored at -20ºC until expiration date.
Thawed medium can be stored at 2 to 8ºC for up to 1 month.
Multiple freeze/thaw cycles are not recommended

Analysis Note

These products are manufactured with the highest quality of raw materials, with exacting standards and production procedures to ensure lot-to-lot consistency. Every lot of medium is extensively tested for the following parameters:

Sterility Testing: Negative for bacterial and fungal growth
pH: 7.5 ± 0.3
Cell Testing: Rate of proliferation and morphology
Osmolality: 255 ± 15 mOsm
Endotoxin: <0.5 EU/mL

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Xeno-free mesenchymal stem cell media specially formulated for the rapid expansion of human mesenchymal stem cells isolated from a variety of sources, including adipose and bone marrow tissues.

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The FibroGRO Xeno-Free Human Fibroblast Expansion Medium is a new variation of our FibroGRO media that is optimized for the culture of fibroblasts without the use of animal-derived components.

description

Defined Xeno-Free osteogenesis differentiation medium that is specially formulated to readily differentiate human mesenchymal stem cells (MSCs) into mature osteocytes as assessed by alizarin red & alkaline phosphatase staining.

description

Defined small molecule based serum-free medium that enables feeder-free culture of human ES/iPS cells and allows for media exchanges every other day without compromising the morphology or long term functionality of human pluripotent stem cells.

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable, cell culture | embryo: suitable

storage temp.

-10 to -25°C

storage temp.

-10 to -25°C

storage temp.

-10 to -25°C

storage temp.

-10 to -25°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Chemicon®

manufacturer/tradename

Millipore, Chemicon®, FibroGRO®

manufacturer/tradename

OsteoMAX-XF

manufacturer/tradename

PluriSTEM®


Storage Class

12 - Non Combustible Liquids



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Questions

  1. Hello, Was the added serum heat-treated? Many thanks!

    1 answer
    1. This product contains 2% Human Serum that is not heat inactivated.

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