Skip to Content
Merck
All Photos(1)

Documents

MABN819

Sigma-Aldrich

Anti-Tau Antibody, oligomeric Antibody, clone TOMA-1

clone TOMA-1, from mouse

Synonym(s):

Microtubule-associated protein tau oligomer, Tau oligomer, PHF-tau oligomer, Paired helical filament-tau oligomer, Neurofibrillary tangle protein oligomer

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TOMA-1, monoclonal

species reactivity

rat, human, mouse

species reactivity (predicted by homology)

mammals (based on high homology)

technique(s)

ELISA: suitable
dot blot: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
neutralization: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... MAPT(4137)
mouse ... Mapt(17762)
rat ... Mapt(29477)

General description

Microtubule-associated protein tau (UniProt P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as TAU, MAPT1, MTBTL) gene (Gene ID 4137) in human. In Alzheimer′s disease (AD) pathology, accumulation of the microtubule-associated protein tau takes place primarily in the neurons. Tau accumulates in both the somatodendritic and axonal domains of neurons. Tau also accumulates in the soma as neurofibrillary tangles (NFTs). Cell death and synaptic lesions occur independently of NFT formation, and research indicates that NFT formation alone is insufficient for neurodegeneration, suggesting that soluble tau aggregates may be the more toxic and pathologically significant tau species. Tau oligomers are neurotoxic when applied extracellularly to cultured neuronal cells, and tau oligomers (but not fibrils) induce neurodegeneration and synaptic and mitochondrial dysfunction in vivo. Moreove, researchers are able to use tau oligomers as a reliable biomarker to differentiate AD brains from age-matched non-AD brains.

Specificity

Clone TOMA-1 (a.k.a. clone H12C10) is among more than 13 hybridomas derived from mice immunized with in vitro generated recombinant Tau-441 (Tau-F, Tau-4, 2N4R isoform) oligomers. These TOMAs are reported to specifically detect oligomeric tau without any significant reactivity toward monomeric tau, tau fibrils, Aβ oligomers, Aβ fibrils, or α-synuclein oligomers (Castillo-Carranza, D.L., et al. (2014). J. Neurosci. 34(12):4260-4272).

Immunogen

Recombinant human Tau-441 (Tau-F, Tau-4, 2N4R isoform) oligomers (Lasagna-Reeves, C. A., et al. (2012). FASEB J. 26(5):1946-1959).

Application

Anti-Tau, oligomeric, clone TOMA-1, Cat. No. MABN819, is amouse monoclonal antibody that targets tau oligomers and has been tested in Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry, Neutralization, and Western Blotting applications.
Research Category
Neuroscience
Western Blotting Analysis: 20 µg/mL from a representative lot detected upregulated oligomeric tau in soluble PBS brain extract from Alzheimer′s diseased (AD) human brain (Courtesy of Sengupta, U., et al., Kayed lab, University of Texas, Galveston).

Please refer to the following publications regarding the use of TOMA clones in Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry, Neutralization, and Western Blotting applications:

1. Vuono, R., et al. (2015). Brain. 138(Pt 7):1907-1918.
2. Castillo-Carranza, D.L., et al. (2015). J. Neurosci. 35(12):4857-4868.
3. Castillo-Carranza, D.L., et al. (2014). J. Neurosci. 34(12):4260-4272.

Quality

Evaluated by Western Blotting in Alzheimer′s diseased human brain lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected Tau oligmers in 25 µg of Alzheimer′s diseased human brain lysate.

Target description

Variable depending on the size(s) of the oligomer(s). Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein A purified.
Purified mouse IgG2aκ in buffer containing PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Yan Zhu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(48), 10255-10270 (2018-10-17)
Brainstem locus ceruleus neurons (LCn) are among the first neurons across the lifespan to evidence tau pathology, and LCn are implicated in tau propagation throughout the cortices. Yet, events influencing LCn tau are poorly understood. Activated persistently across wakefulness, LCn
Fan Xia et al.
Acta neuropathologica communications, 9(1), 51-51 (2021-03-26)
The retina, as the only visually accessible tissue in the central nervous system, has attracted significant attention for evaluating it as a biomarker for neurodegenerative diseases. Yet, most of studies focus on characterizing the loss of retinal ganglion cells (RGCs)
Urmi Sengupta et al.
Methods in molecular biology (Clifton, N.J.), 2754, 147-183 (2024-03-21)
Tau oligomers have been shown to be the main toxic tau species in several neurodegenerative disorders. To study tau oligomers, we have developed reagents and established methods for the reliable preparation, isolation, and detection of tau oligomers as well as
Urmi Sengupta et al.
Annals of clinical and translational neurology, 4(4), 226-235 (2017-04-07)
With an increasing incidence of Alzheimer's disease (AD) and neurodegenerative tauopathies, there is an urgent need to develop reliable biomarkers for the diagnosis and monitoring of the disease, such as the recently discovered toxic tau oligomers. Here, we aimed to
Fang Du et al.
Brain : a journal of neurology, 146(10), 4378-4394 (2023-04-19)
Prolonged exposure to glucocorticoids, the main stress hormones, damages the brain and is a risk factor for depression and Alzheimer's disease. Two major drivers of glucocorticoid-related neurotoxicity are mitochondrial dysfunction and Tau pathology; however, the molecular/cellular mechanisms precipitating these events

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service