AB3841

Anti-Connexin 43 Antibody, phospho-specific (Ser368)

Chemicon^®, from rabbit

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: hamster, mouse, rat, monkey, human
• manufacturer/tradename: Chemicon^®
• technique(s): western blot: suitable
• NCBI accession no.: NM_000165.3
• UniProt accession no.: P17302
• shipped in: dry ice
• target post-translational modification: phosphorylation (pSer368)
• Gene Information: human ... GJA1(2697)

Description

Specificity

Recognizes Ser368 phosphorylated Connexin 43. Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap-junction communication is important in development and regulation of cell growth.

Immunogen

Epitope: phospho-specific (Ser368)

KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser368 of human Connexin 43.

Application

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-Connexin 43 Antibody, phospho-specific (Ser368) is validated for use in WB for the detection of Connexin 43.

Western blotting 1:1,000 (for best results, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight)

Optimal working dilutions must be determined by the end user.

Physical form

Protein A and peptide affinity purified immunoglobulin. Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, with 50% glycerol.

Storage and Stability

Maintain at -20ºC for 12 months after date of receipt. Do not aliquot.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2