189490

3-MA

≥95% (HPLC), powder, autophagy inhibitor, Calbiochem^®

• Synonym(s): Autophagy Inhibitor, 3-MA, 3-Methyladenine
• Empirical Formula (Hill Notation): C₆H₇N₅
• CAS Number: 5142-23-4
• Molecular Weight: 149.15
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD00010531
• Assay: ≥95% (HPLC)
• Form: powder
• Storage condition: OK to freeze, protect from light

Properties

• Product Name: Autophagy Inhibitor, 3-MA, Autophagy Inhibitor, 3-MA, CAS 5142-23-4, is a cell-permeable autophagic sequestration blocker. Acts as an inhibitor of III PI3-Kinase.
• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: white to faint yellow
• solubility: DMSO: 10 mg/mL, water: 5 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: N1(C2=NC=NC2=C(N=C1)N)C
• InChI: 1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3
• InChI key: FSASIHFSFGAIJM-UHFFFAOYSA-N

Description

General description

A widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC₅₀ = 4.5 mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 (Cat. Nos. 440202 and 440204 ) and Wortmannin (Cat. No. 681675 )

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.

In applications where high working concentrations are necessary (e.g. 10 mM), the use of a pre-made stock solution may result in the introduction of higher than desired solvent (e.g., DMSO) into the experimental systems. In such cases, it is recommended that the solid compound be weighed out at the time of use and reconstituted directly into the solutions used in the experimental system (buffers, media, etc.). Vortexing may be required for complete solubilization in water.

Other Notes

Canu, N., et al. 2005. J. Neurochem.92, 1228.
Hirosako, K., et al. 2004. Biochem. Biophys. Res. Commun.316, 845.
Beugnet, A., et al. 2003. Biochem. J.372, 555.
Eskelinen, E.L., et al. 2002. Traffic3, 878.
Petiot, A., et al. 2000. J. Biol. Chem.275, 992.
Seglen, P.O. and Gordon, P.B., 1982. Proc. Natl. Acad. Sci. USA79, 1889.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable