1.50448

SeQuant^® ZIC^®-HILIC 200Å (3.5 µm) HPLC Columns

L × I.D. 150 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

• Synonym(s): Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
• UNSPSC Code: 41115709
• NACRES: NB.21
• Particle size: 3.5 μm
• Matrix active group: ZIC-HILIC, ZIC-HILIC bonding phase
• Pore size: 200 Å pore size
• Matrix: fully porous particle

Properties

• Product Name: SeQuant^® ZIC-HILIC^™ 3.5µm,200Å 150 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
• material: PEEK-lined stainless steel column
• Quality Segment: 100
• agency: suitable for USP L114
• product line: SeQuant^®
• feature: endcapped: no
• analyte functional class(es): antibiotics, sweeteners, water soluble vitamins
• analyte chemical class(es): PFAS, alcohols / fermentation, aliphatic amines, alkaloids, amino acids, aromatic amines, carboxylic acids as methyl esters, esters, glycans, glycols, inorganic ions, monosaccharides, nitrosamines, nucleosides, oligonucleotides, organic phosphates, peptides, pesticides, phospholipids, polysaccharides, small carbohydrates, sugar alcohols, sulfonamides
• parameter: 550 bar pressure, 70 °C max. temp.
• technique(s): HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)
• column L × I.D.: 15 cm × 2.1 mm
• matrix: fully porous particle
• matrix active group: ZIC-HILIC, ZIC-HILIC bonding phase
• particle size: 3.5 μm
• pore size: 200 Å pore size
• operating pH: 2-7.5
• separation technique: hydrophilic interaction (HILIC)
• storage temp.: no temp limit

Description

General description

SeQuant^® ZIC^®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 150 mm length x 2.1 mm ID is the optimal choice for the separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant^® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^® ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Application

• Click postsynthesis of microporous organic network@silica composites: Demonstrates the application of SeQuant ZIC-HILI columns in mixed-mode chromatography, particularly in the synthesis of composites that facilitate both reversed-phase and hydrophilic interaction chromatography. This approach is crucial for advanced pharmaceutical compound analysis and biomolecule separation technology (Sun HF et al., 2023).


• Evaluation of Hold-Up Volume Determination Methods and Markers in Hydrophilic Interaction Liquid Chromatography: Focuses on refining the precision in volume determinations using ZIC-HILIC columns, enhancing method development for pharmaceuticals and providing a basis for high-resolution mass spectrometry applications (Redon L et al., 2023).


• Determination of the polyphenolic content of berry juices: Utilizes SeQuant ZIC-HILIC technology for comprehensive two-dimensional liquid chromatography, essential for complex sample analysis in food safety and quality assessments (Arena K et al., 2023).


• Simple and sensitive determination of sulfites in Chinese herbal teas: Highlights the robustness of SeQuant ZIC-HILIC in ultra-high-performance liquid chromatography tandem mass spectrometry, ensuring the safety and compliance of herbal products with stringent health standards (Yang L et al., 2022).

Analysis Note

Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.40

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany