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07-641

Sigma-Aldrich

Anti-MEK1 Antibody

Upstate®, from rabbit

Synonym(s):

Anti-CFC3, Anti-MAPKK1, Anti-MEK1, Anti-MEL, Anti-MKK1, Anti-PRKMK1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_002755

UniProt accession no.

Q02750

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... MAP2K1(5604)

Related Categories

Specificity

MEK1
Predicted to cross-react with rabbit based on sequence homology.

Immunogen

Peptide corresponding to amino acids 2-18 (PKKKPTPIQLNPAPDGS) of human MEK1.

Application

Research Category
Signaling
Research Sub Category
MAP Kinases
This Anti-MEK1 Antibody is validated for use in IP, WB for the detection of MEK1.

Quality

Routinely evaluated by immunoblot on RIPA lysates from A431 and 3T3/A31 cells.

Target description

45kDa

Linkage

Replaces: 04-376

Physical form

70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol.
Format: Purified
Protein A purified

Storage and Stability

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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