Saltar al contenido
Merck

56401AST

Supelco

apHera NH₂ (5 μm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Sinónimos:

Amino HPLC Column

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Columna para HPLC apHera NH2, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Quality Level

agency

suitable for USP L82

product line

apHera

packaging

pkg of 1 ea

manufacturer/tradename

apHera

parameter

147 bar pressure (2133 psi)
4-50 °C temperature

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

matrix

polymeric

matrix active group

amino phase

particle size

5 μm

pore size

300 Å

operating pH

1-14

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

¿Está buscando productos similares? Visita Guía de comparación de productos

General description

apHera amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides. The elution order mono-, di-, tri-saccharide shows increased eluton volume with increased acetonitrile concentration and complete stability for both acidic and alkaline eluates. The small, robust PVA copolymer bead provides mechanical and chemical strength as well as high column efficiency. Conventional camion columns based on silica do not show long column life, perhaps due to hydrolysis of silica particle by the basic amino group. Since Supelco uses a strong alkaline compatible polymer, these problems are eliminated. Stable retention time and long column life are also characteristic of the column.

Features of Amino apHera Columns
  • High efficiency for carbohydrate analysis
  • Ideal for basic conditions/amphoteric detection
  • High selectivity mono- to oligosaccharides
  • pH range 2-13
  • 300 angstrom pore size
  • Stable, predictable retention
  • Long column life

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

apHera is a trademark of Sigma-Aldrich Co. LLC

guard column

Referencia del producto
Descripción
Precios

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Khin Than Myint et al.
Analytical chemistry, 81(18), 7766-7772 (2009-08-26)
We have developed a practical method for the comprehensive analysis of polar anionic metabolites in biological samples with the use of a nano-LC/MS system. A polyamine-bonded polymer-based apHera NH2 column, which is compatible with ammonium carbonate buffer, effectively retained anionic
Marine de Person et al.
Journal of chromatography. A, 1081(2), 174-181 (2005-07-26)
A new procedure based on hydrophilic interaction chromatography coupled to tandem mass spectrometry (ionisation process by pneumatically assisted electrospray in negative ion mode), is developed and validated for the simultaneous determination of underivatised taurine and methionine in beverages rich in

Artículos

The apHera NH2 polymeric column is an excellent choice for the separation of closely-related polar analytes such as simple sugars using HILIC chromatography.

Glyphosate and related compounds are measured in oatmeal and infant cereal using ion-exchange polymer-based particles for HPLC and SPE. Low level detection was obtained.

Glyphosate and related compounds are measured in oatmeal and infant cereal using ion-exchange polymer-based particles for HPLC and SPE. Low level detection was obtained.

Glyphosate and related compounds are measured in oatmeal and infant cereal using ion-exchange polymer-based particles for HPLC and SPE. Low level detection was obtained.

Ver todo

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico