Unfortunately, this product has not been sequenced. In general, Ubiquitin is fairly conserved across species. Please see the link below to review a publication that reports that human and bovine ubiquitin are identical:
https://www.nature.com/articles/255423a0
U6253
Ubiquitin from bovine erythrocytes
BioUltra, ≥98% (SDS-PAGE), essentially salt-free, lyophilized powder
Sinónimos:
ATP-dependent proteolytic factor, Ub
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About This Item
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
biological source
bovine erythrocytes
Quality Level
product line
BioUltra
assay
≥98% (SDS-PAGE)
form
essentially salt-free, lyophilized powder
storage condition
(Tightly closed. Dry)
technique(s)
western blot: suitable
impurities
salt, essentially free
solubility
water: 1 mg/mL, clear, colorless
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... LOC(101902760)
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Categorías relacionadas
General description
Research area: Cancer
Ubiquitin is a highly conserved regulatory protein. It is found in all eukaryotic cells and is virtually identical across all forms of life including yeast, humans, and plants. ubiquitin structure contains seven Lys residues and an N-terminus, all of which are target sites for ubiquitination.[1]
Ubiquitin is a highly conserved regulatory protein. It is found in all eukaryotic cells and is virtually identical across all forms of life including yeast, humans, and plants. ubiquitin structure contains seven Lys residues and an N-terminus, all of which are target sites for ubiquitination.[1]
This product is from bovine red blood cells. It is not a recombinant product. The production process is considered proprietary, however it includes heat treatment as well as purification by chromatography and dialysis.
Application
Ubiquitin from bovine erythrocytes has been used to study the role of exogenous ubiquitin in chronic β-adrenergic receptor (β-AR)-stimulated myocardial remodeling.[2] It has also been used to test the inhibitor of nuclear factor kappa-B kinase subunit beta (IKKβ) ubiquitylation.[3]
Ubiquitin from bovine erythrocytes can be used for in vitro ubiquitinylation assay.[4] The product can also be used as a marker in western blotting.[5]
Ubiquitin from bovine erythrocytes can be used for in vitro ubiquitinylation assay.[4] The product can also be used as a marker in western blotting.[5]
Biochem/physiol Actions
Ubiquitination is a post-translational modification process where ubiquitin-protein is attached to a substrate protein. Ubiquitination plays a vital role in the regulation of cellular signaling in various biological processes such as apoptosis, protein processing, immune response, and DNA repair. Ubiquitination mediates protein degradation via the ubiquitin-proteasome pathway. Ubiquitination is implicated in various cellular signaling pathways. Polyubiquitination modulates the signal activation of NF-κ-B inhibitor alpha (IkB-α) in the inflammatory signaling pathway.[1] Elevated levels of ubiquitin have been observed in various diseases such as parasitic and allergic diseases, alcoholic liver disease, type 2 diabetes, β2-microglobulin amyloidosis, and chronic hemodialysis.[2]
Preparation Note
Ubiquitin from bovine erythrocytes can dissolved in water at 1 mg/ml to yield a clear, colorless solution.
antibody
Referencia del producto
Descripción
Precios
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
Lot/Batch Number
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Los clientes también vieron
Takeshi Miura et al.
FEMS microbiology letters, 239(1), 171-179 (2004-09-29)
When Saccharomyces cerevisiae cells are exposed to high hydrostatic pressure, tryptophan permease Tat2 is degraded in a manner dependent on Rsp5 ubiquitin ligase. Consequently, cell growth is arrested in tryptophan auxotrophic strains. Here we show that of 17 ubiquitin-specific protease
The ubiquitin system for protein degradation.
A Hershko et al.
Annual review of biochemistry, 61, 761-807 (1992-01-01)
A L Schwartz et al.
Annual review of medicine, 50, 57-74 (1999-03-12)
The ubiquitin-proteasome pathway plays a pivotal role in the degradation of short-lived and regulatory proteins important in a variety of basic cellular processes, including regulation of the cell cycle, modulation of cell surface receptors and ion channels, and antigen presentation.
Biochemistry, Ubiquitination
Guo HJ, et al.
StatPearls [Internet] (2022)
Christopher R Daniels et al.
American journal of physiology. Heart and circulatory physiology, 303(12), H1459-H1468 (2012-10-09)
β-Adrenergic receptor (β-AR) stimulation increases extracellular ubiquitin (UB) levels, and extracellular UB inhibits β-AR-stimulated apoptosis in adult cardiac myocytes. This study investigates the role of exogenous UB in chronic β-AR-stimulated myocardial remodeling. l-Isoproterenol (ISO; 400 μg·kg(-1)·h(-1)) was infused in mice
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The uniprot id provided on this website is for human poly Ubiquitin. Can you tell me the exact sequence of monoubiquitin that you have purified from bovine erythrocytes?
1 answer-
Helpful?
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Could you provide information about potential chemical residue in the product? Specifically, I would like to know if ethanol and/or acetone were used during the isolation and production process. I have detected some candidate adducts from this product and need to exclude the possibility that they originated from an artificial source.
1 answer-
The available testing information is limited to what is provided on the Certificate of Analysis (CofA). The specific method of production is considered proprietary; however, some general information about the production process can be shared. The details are as follows:
The final product should not contain any additives or stabilizers. The process involves overnight dialysis with deionized water to remove any remaining salts or additives. Subsequently, the product is removed from dialysis, filtered, and lyophilized. It is improbable that any ethanol or acetone would remain after the lyophilization process.
Helpful?
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I would like to know how this Ubiquitin is expressed and purified.
1 answer-
This product is from bovine red blood cells. It is not a recombinant product. The production process is considered proprietary, however it includes heat treatment as well as purification by chromatography and dialysis.
Helpful?
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