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R9379

Sigma-Aldrich

Rhodamine B isothiocyanate–Dextran

average mol wt ~70,000

Sinónimos:

RITC-Dextran

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About This Item

MDL number:
UNSPSC Code:
12171501
NACRES:
NA.56

mol wt

average mol wt ~70,000

Quality Level

extent of labeling

0.002-0.015 mol RITC per mol glucose

storage temp.

2-8°C

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General description

Rhodamine B is a thiol-reactive and photostable fluorescent dye.

Application

Rhodamine B isothiocyanate-Dextran has been used:
  • to label A, CI-M6PR-GFP, Lamp1-CFP-expressing wild type mouse embryonic fibroblast (MEF) cells
  • in endosome/phagosome fusion assay to determine the rate of fusion of endosomes
  • as a component in poration medium to monitor Hela cell uptake

Dextran labeled with rhodamine B isothiocyanate for possible use in perfusion studies in animals.

Biochem/physiol Actions

Rhodamine B stains the tissue in red-violet color and emits a bright red color when fluoresced. It is highly preferred for marking insects.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Francesca Ungaro et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 41(1), 60-70 (2010-06-01)
Gas-foamed large porous particles (gfLPP) based on poly(lactic-co-glycolic) acid (PLGA) have been recently suggested as potential carriers for pulmonary drug delivery. In this work, we attempt to engineer gfLPP for efficient local delivery of macromolecules in the lungs. Particles were
Pascale Vérant et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 27(5), 1072-1081 (2006-10-26)
Knowledge of the blood volume per unit volume of brain tissue is important for understanding brain function in health and disease. We describe a direct method using two-photon laser scanning microscopy to obtain in vivo the local capillary blood volume
Xiuqing Gong et al.
Lab on a chip, 10(19), 2622-2627 (2010-08-07)
We report a simple, low-cost and detachable microfluidic chip incorporating easily accessible paper, glass slides or other polymer films as the chip materials along with adhesive wax as the recycling bonding material. We use a laser to cut through the
Shikha Prasad et al.
Redox biology, 12, 58-69 (2017-02-18)
Cigarette smoking (CS) is associated with vascular endothelial dysfunction in a causative way primarily related to the TS content of reactive oxygen species (ROS), nicotine, and inflammation. TS promotes glucose intolerance and increases the risk of developing type-2 diabetes mellitus
Atg5 regulates late endosome and lysosome biogenesis
Peng JY, et al.
Science China: Life Sciences, 57(1), 59-68 (2014)

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