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R4526

Sigma-Aldrich

Reference Dye for Quantitative PCR

100 ×, solution

Sinónimos:

Reference dye for qPCR, ROX

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About This Item

UNSPSC Code:
41106300
NACRES:
NA.55

form

solution

Quality Level

300

usage

sufficient for ≥600 reactions

concentration

100 ×

technique(s)

PCR: suitable

color

red

solubility

water: soluble

storage temp.

2-8°C

Categorías relacionadas

General description

Sigma′s Reference Dye for quantitative polymerase chain reaction (qPCR) is a proprietary dye for use with real-time PCR. It is used for the normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.

Application

Reference Dye for Quantitative PCR has been used:
  • in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR)
  • as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)
  • for analyzing the degree of cellular DNA contamination by qPCR

Other Notes

Reference Dye for Quantitative PCR is forR&D use only. Not for drug, household, or other uses.

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Referencia del producto
Descripción
Precios

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Ryan Manow et al.
Journal of industrial microbiology & biotechnology, 39(7), 977-985 (2012-03-01)
Previously, a native homoethanol pathway was engineered in Escherichia coli B by deletions of competing pathway genes and anaerobic expression of pyruvate dehydrogenase (PDH encoded by aceEF-lpd). The resulting ethanol pathway involves glycolysis, PDH, and alcohol dehydrogenase (AdhE). The E.
Partial deletion of rng (RNase G)-enhanced homoethanol fermentation of xylose by the non-transgenic Escherichia coli RM10
Manow R, et al.
Journal of Industrial Microbiology & Biotechnology, 39(7), 977-985 (2012)
Sambrook, J. et al.
Molecular Cloning: A Laboratory Manual, 3rd (2000)
Ryan Manow et al.
World journal of microbiology & biotechnology, 36(4), 59-59 (2020-04-03)
An endogenous homoethanol pathway (glucose/1.2 xylose => 2 pyruvate => 2 ethanol) was previously engineered in Escherichia coli SZ410 via eliminating acid-producing pathways and anaerobic expression of the pyruvate dehydrogenase complex (aceEF-lpd operon). This ethanologenic derivative was subsequently engineered through adaptive evolution and partial
Kevin Antoine Brown et al.
Infection control and hospital epidemiology, 39(8), 917-923 (2018-08-10)
Clostridium difficile spores play an important role in transmission and can survive in the environment for several months. Optimal methods for measuring environmental C. difficile are unknown. We sought to determine whether increased sample surface area improved detection of C.
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Protocolos

SYBR® Green JumpStart™ Taq ReadyMix™

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

Universal SYBR Green qPCR Protocol

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

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Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

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