This product is an ammonium sulfate suspension. This is an enzyme, and batch-specific Certificates of Analysis report the activity of the enzyme. Most customers use it as an active enzyme. Please reach out to local technical support for clarification on using the inactivated form of the enzyme.
R1003
Ribonuclease T1 from Aspergillus oryzae
ammonium sulfate suspension, 300,000-600,000 units/mg protein
Sinónimos:
Guanyloribonuclease, Ribonucleate 3′-guanylo-oligonucleotidohydrolase
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About This Item
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Productos recomendados
origen biológico
Aspergillus sp. (Aspergillus oryzae)
Nivel de calidad
Formulario
ammonium sulfate suspension
actividad específica
300,000-600,000 units/mg protein
mol peso
11068 by amino acid sequence
técnicas
cell based assay: suitable
idoneidad
suitable for separating native or denatured proteins, or nucleic acids
aplicaciones
cell analysis
temp. de almacenamiento
2-8°C
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Aplicación
Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies [1].
Acciones bioquímicas o fisiológicas
Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3′-phosphate group of a guanidine ribonucleotide and 5′-hydroxyl of the adjacent nucleotide. The initial product is a 2′:3′ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3′-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group.
Definición de unidad
One unit will produce acid soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 15 min at pH 7.5 at 37°C, in a reaction volume of 1.0 mL. Substrate: Yeast RNA.
Forma física
Suspension in 2.8 M (NH4)2SO4 solution
Nota de análisis
Protein determined by E1%/280
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves
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Certificados de análisis (COA)
Lot/Batch Number
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Los clientes también vieron
Ribonuclease T1: Structure, Function, and Stability
Pace, CN; Heinemann U, et al.
Angewandte Chemie (International Edition in English), 4, 343-360 (1991)
Rasmus Lybech Jensen et al.
Journal of the American Chemical Society, 134(23), 9820-9826 (2012-05-19)
Singlet molecular oxygen, O(2)(a(1)Δ(g)), can influence many processes pertinent to the function of biological systems, including events that result in cell death. Many of these processes involve a reaction between singlet oxygen and a given amino acid in a protein.
Herry Martadinata et al.
Biochemistry, 50(29), 6455-6461 (2011-06-16)
The discovery of long RNA transcripts of telomeric repeats (TERRA) and their potential to form G-quadruplexes stimulated studies on the possible arrangements of G-quadruplexes along TERRA. Here we performed ribonuclease protection assay to investigate the structures formed by long human
C Nick Pace et al.
Journal of molecular biology, 408(3), 514-528 (2011-03-08)
Our goal was to gain a better understanding of the contribution of hydrophobic interactions to protein stability. We measured the change in conformational stability, Δ(ΔG), for hydrophobic mutants of four proteins: villin headpiece subdomain (VHP) with 36 residues, a surface
Scott Quarrier et al.
RNA (New York, N.Y.), 16(6), 1108-1117 (2010-04-24)
Structure mapping experiments (using probes such as dimethyl sulfate [DMS], kethoxal, and T1 and V1 RNases) are used to determine the secondary structures of RNA molecules. The process is iterative, combining the results of several probes with constrained minimum free-energy
Artículos
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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This product was in powder form before and is now in liquid form. Do you still have to inactivate 20 min at 80°C before use? Thank you very much
1 answer-
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Is it possible to store this product frozen at -20°C?
1 answer-
Storing ammonium sulfate suspensions of proteins frozen can lead to the removal of ammonium sulfate from the protein crystal structure. This exposes the protein to a high amount of ammonium sulfate, potentially causing denaturation due to high salt content. Consequently, the enzyme activity may decrease, as may have occurred in this instance. It is recommended that the customer purchase a new bottle and store it at 2-8°C.
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