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Merck

P8849

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic, aminopeptidases and thermolysin-like activities, for use in purification of Histidine-tagged proteins, DMSO solution

Sinónimos:

Protease inhibitor solution

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About This Item

Número MDL:
Código UNSPSC:
12352200
NACRES:
NA.77
Formulario:
DMSO solution
solubilidad:
water: soluble
temp. de almacenamiento:
−20°C
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nombre del producto

Cóctel de inhibidores de proteasas, for use in purification of Histidine-tagged proteins, DMSO solution

origen biológico

synthetic

Nivel de calidad

Formulario

DMSO solution

solubilidad

water: soluble

temp. de almacenamiento

−20°C

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Descripción general

DMSO solution designed for use in the purification of histidine-tagged proteins.

The cocktail contains a mixture of inhibitors that specifically target serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Especificidad

Inhibits serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Aplicación

The cocktail has been optimized and tested for isolation of histidine-tagged proteins, with chelating agents omitted for compatibility with IMAC applications.

It is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells.

Características y beneficios

Specifically formulated for histidine-tagged protein purification.

Targets multiple types of proteases, ensuring comprehensive inhibition of protease activity.

Compatibility with IMAC applications due to omission of chelating agents.

Supplied in convenient packaging options.

Componentes

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon

Cantidad

One mL is recommended for the inhibition of proteases extracted from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells in a total volume of 100 ml.

This protease inhibitor cocktail has been optimized and tested for histidine-tagged proteins

Nota de preparación

This product is supplied as a clear solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of E. coli cells or 10 g of baculovirus-infected cells.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

185.0 °F - closed cup

Punto de inflamabilidad (°C)

85 °C - closed cup


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Z Kurowska et al.
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E Boudreau et al.
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Shuji Kaneko et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(1), 82-92 (2002-01-05)
The physical interaction between the presynaptic vesicle release complex and the large cytoplasmic region linking domains II and III of N-type (Ca(v)2.2) calcium channel alpha(1)B subunits is considered to be of fundamental importance for efficient neurotransmission. By PCR analysis of
Ryan McKay et al.
Biotechnology and bioengineering, 114(12), 2883-2895 (2017-07-30)
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Thomas A Leonard et al.
Cell, 144(1), 55-66 (2011-01-11)
Protein kinase C (PKC) isozymes are the paradigmatic effectors of lipid signaling. PKCs translocate to cell membranes and are allosterically activated upon binding of the lipid diacylglycerol to their C1A and C1B domains. The crystal structure of full-length protein kinase

Contenido relacionado

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Questions

1–8 of 8 Questions  
  1. Is it 100x?

    1 answer
    1. While not specifically listed as a '100X' concentration, a volume of 1 ml of this solution is sufficient for 100ml of cell lysate. However, this is based on the mass and origin of the lysate preparation. The 100 mL of cell lysate referenced is prepared from 20 g of E. coli cells or 10 g of baculovirus-infected cells. Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/934/419/p8849dat-ms.pdf

      Helpful?

  2. What is the stability at -20°C of the protease inhibitor cocktail in DMSO?

    1 answer
    1. This product is quite stable when stored at the recommended -20°C. The material is not assigned an expiration date but has a recommended retest date of 4 years from the date of quality release.

      Helpful?

  3. Is there any miscibility data available for Cat # P8849?

    1 answer
    1. P8849 is DMSO based, and DMSO is known to be miscible in water and cell culture media. The product is supplied as a clear solution in DMSO, with 1 mL of solution.

      Helpful?

  4. Can this product be used in Expi293 mammalian cell culture for stability of secreted proteins without harming the cells during expression or is this recommended only after cells spun out of media containing 6-His tagged proteins?

    1 answer
    1. This product has not been tested for use in cell culture medium as an additive to preserve secreted proteins. Please see the link below to review an alternative product tested specifically for this application:
      P1860 Datasheet
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/413/261/p1860dat-ms.pdf

      P1860 Product Page
      https://www.sigmaaldrich.com/product/sigma/p8340

      Helpful?

  5. How much of Product No. P8849, Protease Inhibitor Cocktail, should be added to the cell lysate?

    1 answer
    1. A 1:100 dilution is the recommended concentration.

      Helpful?

  6. Can Product No. P8849, Protease Inhibitor Cocktail, be used with with HIS-Select™ products or any other immobilized metal affinity chromatography (IMAC) products?

    1 answer
    1. This product does not contain EDTA and can be used with sample processing by immobilized metal affinity chromatography (IMAC) suchas used for the purification of histidine-tagged proteins..

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. What is the difference between Protease Inhibitors P8849 and S8830?

    1 answer
    1. P8849 is a DMSO solution and S8830 is a water soluble tablet. P8849 formulation is considered proprietary but does contain AEBSF, Pepstatin A, Bestatin, E-64, and Phosphoramidon. The formulation of S8830 also contains Aprotinin and Pepstatin A and the exact formulation is given on the product information sheet (under Documents, above).

      Helpful?

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