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Merck

P2860

Sigma-Aldrich

Monoclonal Anti-PSF antibody produced in mouse

clone B92, purified from hybridoma cell culture

Sinónimos:

Mouse Anti-Polypyrimidine tract-binding protein-associated splicing factor

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

B92, monoclonal

Formulario

buffered aqueous solution

reactividad de especies

mouse, human

envase

antibody small pack of 25 μL

concentración

~2 mg/mL

técnicas

immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
radioimmunoassay: suitable using cells
western blot: 2-4 μg/mL using whole extract of cultured HeLa cells

isotipo

IgG1

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... TAP1(6890)

Descripción general

Monoclonal Anti-PSF (mouse IgG1 isotype) is derived from the B92 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a C57BL/6J mouse immunized with a lysate of mouse bone marrow-derived stromal cell line. Polypyrimidine tract-binding protein-associated splicing factor (PSF) is an ubiquitous nuclear matrix protein. It is characterized with two consensus RNA-binding domains and an unusual amino terminus rich in proline and glutamine residues.

Inmunógeno

lysate of mouse bone marrow-derived stromal cell line.

Aplicación

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunoprecipitation (1 paper)
Monoclonal Anti-PSF antibody produced in mouse has been used in:
  • immunofluorescence
  • immunohistochemistry
  • immunoprecipitation

Acciones bioquímicas o fisiológicas

Polypyrimidine tract-binding protein-associated splicing factor (PSF) plays a pivotal role in mammalian spliceosome. PSF is also necessary for pre-mRNA splicing and transcription. The restricted expression of PSF suggests that it is involved in the control of neuronal-specific splicing events occurring at particular stages of neuronal differentiation and maturation. Monoclonal antibodies reacting specifically with PSF are useful tools for the molecular identification and characterization of the functional activity of PSF.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Nota de preparación

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Wen Shen et al.
Nucleic acids research, 46(5), 2204-2217 (2018-02-02)
We reported previously that a 2' fluoro-modified (2' F) phosphorothioate (PS) antisense oligonucleotides (ASOs) with 5-10-5 gapmer configuration interacted with proteins from Drosophila behavior/human splicing (DBHS) family with higher affinity than PS-ASOs modified with 2'-O-(2-methoxyethyl) (2' MOE) or 2',4'-constrained 2'-O-ethyl
Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice
Nakagawa S, et al.
The Journal of cell biology, 193(1), 31-39 (2011)
Cytoplasmic Relocalization of TAR DNA-Binding Protein 43 Is Not Sufficient to Reproduce Cellular Pathologies Associated with ALS In vitro
Wobst HJ, et al.
Frontiers in Molecular Neuroscience, 10(7) (2017)
Chungoo Park et al.
Scientific reports, 7(1), 11211-11211 (2017-09-13)
Rbfox RNA-binding proteins play important roles in the regulation of alternative pre-mRNA splicing, but their role in other gene regulatory mechanisms is not well understood. Here, we show that Rbfox2 is a novel constituent of cytoplasmic stress granules, the translational
Differential nuclear localization and nuclear matrix association of the splicing factors PSF and PTB
Meissner M, et al.
Journal of Cellular Biochemistry, 76(4), 559-566 (2000)

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