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M6900

Sigma-Aldrich

Methyl Blue

powder or crystals

Sinónimos:

Acid blue 93, Aniline blue water soluble, Cotton blue, Poirriers blue, Water blue

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About This Item

Fórmula empírica (notación de Hill):
C37H27N3Na2O9S3
Número de CAS:
28983-56-4
Peso molecular:
799.80
Colour Index Number:
42780
EC Number:
249-352-9
MDL number:
MFCD00058509
UNSPSC Code:
12171500
PubChem Substance ID:
329818037
NACRES:
NA.47

form

powder or crystals

Quality Level

200

mp

>-23 °C

solubility

H2O: 1 mg/mL, blue

εmax

≥650 at 594-610  nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].OS(=O)(=O)c1ccc(Nc2ccc(cc2)\C(c3ccc(Nc4ccc(cc4)S([O-])(=O)=O)cc3)=C5/C=CC(\C=C5)=N/c6ccc(cc6)S([O-])(=O)=O)cc1

InChI

1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2

Inchi Key

MCPLVIGCWWTHFH-UHFFFAOYSA-L

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General description

Methyl Blue is a triaminotriphenylmethane dye, widely used in paper, textile and leather production.

Application

Methyl Blue has been used:
  • to stain leaf disks for callose visualization within the cells
  • to incubate cells for 4′,6-diamidino-2-phenylindole (DAPI)/septa staining
  • as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin

Biochem/physiol Actions

Methyl Blue is considered as a xenobiotic compound and has antiseptic property. It is useful in polychrome staining. Methyl blue is mostly used for staining samples in histology and microbiology. It is also useful as a model to reveal the role of catalyst in light induced degradation of dyes.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 2

flash_point_f

>572.0 °F - Pensky-Martens closed cup

flash_point_c

> 300 °C - Pensky-Martens closed cup

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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A novel a-1, 3-glucan elicits plant defense responses in potato and induces protection against Rhizoctonia solani AG-3 and Fusarium solani f. sp. eumartii.
Wolski E
Physiological & Molecular Plant Pathology, 69, 93-93 (2006)
A new principle in polychrome staining: a system of automated staining, complementary to hematoxylin and eosin, and usable as a research tool
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Stain Technology, 58(5), 245-258 (1983)
Micellar effect on the kinetics of oxidation of methyl blue by Ce (IV) in sulfuric acid medium
Hassan M, et al.
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Replication stress in early S phase generates apparent micronuclei and chromosome rearrangement in fission yeast
Sabatinos SA, et al.
Molecular Biology of the Cell, 26(19), 3439-3450 (2015)
A novel alpha-1, 3-glucan elicits plant defense responses in potato and induces protection against Rhizoctonia solani AG-3 and Fusarium solani f. sp. eumartii
Wolski EA, et al.
Physiological and Molecular Plant Pathology, 69(1-3), 93-103 (2006)
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