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H0290

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Hartman′s Fixative

histological tissue fixative

Sinónimos:

Davidson′s Fixative

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About This Item

Código UNSPSC:
12171500
NACRES:
NA.47

Nivel de calidad

Formulario

liquid

aplicaciones

hematology
histology

temp. de almacenamiento

room temp

Descripción general

Hartman′s fixative, which is also known as Davidson′s Fixative, contains acetic acid, alcohol, and formalin. It is widely used for the preservation of eye, bone marrow, breast, testis, and other tissues for histologic examination.

Aplicación

An overnight fixative used for the visualization of lymph nodes in radical dissection specimens. It is helpful in visualizing both both breast and colon specimens by turning lymph nodes white. Moore and Barr used a version of Hartman′s fixative for fixing skin biopsy specimens for study of fine nuclear details. Rat testes for immunohistochemical and morphological comparison were fixed using conventional and modified Hartman′s fixative for 24 hours, while eyes and testes for other analyses were fixed for 48 hours.

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1 - Skin Sens. 1 - STOT SE 3

Órganos de actuación

Central nervous system, Respiratory system

Código de clase de almacenamiento

3 - Flammable liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

75.2 °F

Punto de inflamabilidad (°C)

24 °C


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John R Latendresse et al.
Toxicologic pathology, 30(4), 524-533 (2002-08-22)
Most recent revisions of regulatory guidelines for testing effects of chemicals on reproduction recommend Bouin's fluid (BF) or a "comparable fixative" instead of formalin to preserve the morphologic detail of testes for histopathological evaluation. However, picric acid in BF is
Fixation of testes and eyes using a modified Davidson's fluid: comparison with Bouin's fluid and conventional Davidson's fluid.
Latendresse J R, et al.
Toxicologic Pathology, 30(4), 524-533 (2002)
Dan Wang et al.
Biology of reproduction, 102(5), 1033-1044 (2020-02-11)
Fertilization is one of the fundamental biological processes, but so far, we still do not have a full understanding of the underlying molecular mechanism. We have identified a human acrosome protein, LY6/PLAUR domain containing 4 (LYPD4), expressed specifically in human
Generation and functional characterization of a conditional Pumilio2 null allele.
Lin K, et al.
Journal of biomedical research (2017)
The sex chromatin in human malignant tissues.
K L MOORE et al.
British journal of cancer, 11(3), 384-390 (1957-09-01)

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