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F8649

Sigma-Aldrich

Formate Dehydrogenase from Candida boidinii

lyophilized powder, 5.0-15.0 units/mg protein

Sinónimos:

FDH, Formate:NAD+ oxidoreductase

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About This Item

Número de CAS:
9028-85-7
Comisión internacional de enzimas:
1.2.1.2 (BRENDA, IUBMB)
Número CE:
232-844-2
Número MDL:
MFCD00081614
Código UNSPSC:
12352204
NACRES:
NA.54
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

fungus (Candida boidinii)

Nivel de calidad

200

Formulario

lyophilized powder

actividad específica

5.0-15.0 units/mg protein

composición

Protein, 5.0-20.0% biuret

temp. de almacenamiento

2-8°C

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Aplicación

Formate Dehydrogenase (FDH) is used for diagnostics in large scale industrial processes. Its used in the production of an unnatural amino acid, tert-L-leucine, a component of some HIV protease and matrix metalloprotease inhibitors.[1]

Acciones bioquímicas o fisiológicas

Formate dehydrogenase is an abundant enzyme from yeast Candida boidinii (CbFDH) that plays an important role in the energy supply of methylotrophic microorganisms and in the stress response of plants.[1]
Formate dehydrogenase is involved in the stress response of plants [2] and catalyzes the reduction of NAD+ to NADH.[3]

Definición de unidad

One unit will oxidize 1.0 μmole of formate to CO2 per min in the presence of β-NAD at pH 7.6 at 37 °C.

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334

Consejos de prudencia

P261 - P284 - P501

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
SLCG4161
SLCF4323
SLCF0381
SLCD5061
SLCD2542

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Do reactive oxygen species or does oxygen itself confer obligate anaerobiosis? The case of Bacteroides thetaiotaomicron.
Khademian, et al.
Molecular Microbiology, 114, 333-347 (2021)
Held in police custody.
A Skelt
Nursing times, 84(4), 50-52 (1988-01-02)
Jana Löwe et al.
Scientific reports, 8(1), 10436-10436 (2018-07-12)
A biotechnological process is reported, which enables an enzymatic reduction without the need for addition of an organic co-substrate for in situ-cofactor recycling. The process is based on merging the fields of enzymatic reductive amination with formate dehydrogenase-based in situ-cofactor
Yuan Lu et al.
Applied biochemistry and biotechnology, 167(4), 732-742 (2012-05-18)
To improve the hydrogen productivity and examine the hydrogen evolution mechanism of Clostridium paraputrificum, roles of formate in hydrogen evolution and effects of introducing formate-originated NADH regeneration were explored. The formate-decomposing pathway for hydrogen production was verified to exist in
V I Tishkov et al.
Biochemistry. Biokhimiia, 69(11), 1252-1267 (2005-01-04)
NAD+-dependent formate dehydrogenase (FDH) is an abundant enzyme that plays an important role in energy supply of methylotrophic microorganisms and in response to stress in plants. FDH belongs to the superfamily of D-specific 2-hydroxy acid dehydrogenases. FDH is widely accepted
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Questions

1–4 of 4 Questions  

  1. Could you please provide the uniprot accession code of the enzyme? Best regards

    1 answer

    1. The UniProt accession ID is O13437. Please access this link below:
      https://www.uniprot.org/uniprotkb/O13437/entry.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. What solvent can Product F8649, Formate dehydrogenase from Candida boidinii be  dissolved in?

    1 answer

    1. Product F8649, Formate dehydrogenase from Candida boidinii can be dissolved in 50 mM phosphate buffer, pH 7.5 to 8.0, or in double distilled water. Solubility is approximately 10 mg/ml.

      Helpful?

  4. How do you store Formate Dehydrogenase from Candida boidinii , product F8649, once it has been solublized?

    1 answer

    1. Aliquots of the enzyme Formate Dehydrogenase from Candida boidinii , product F8649, should be stable for at least 6 months when stored at -15 to -25°C, at +33°C for 1 day, and at +2 to +8°C for 7 days. Aliquots should be thawed only once.

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