If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
D5941
Drabkin′s reagent
for the determination of hemoglobin
Sinónimos:
Hemoglobin Quantitation Reagent
About This Item
Productos recomendados
Nivel de calidad
Formulario
liquid
Descripción general
Aplicación
Componentes
Producto relacionado
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 2 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - STOT RE 2
Órganos de actuación
Thyroid
Riesgos supl.
Código de clase de almacenamiento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
¿No ve la versión correcta?
Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
-
How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
-
-
How does freezing affect the stability and shelf life of Drabkin's Solution? Would freezing help maintain its efficacy for a longer duration?
1 answer-
The stability of the Drabkin's solution when stored frozen has not been evaluated. The prepared solution is stable for at least 6 months at room temperature when stored protected from light. While it is possible that frozen storage would not impact the quality, this cannot be guaranteed. Please see the link below to review a publication that may be helpful.
'The effect of freezing on the potassium ferricyanide-potassium cyanide reagent used in the cyanmethemoglobin procedure for hemoglobin determination'
https://www.sciencedirect.com/science/article/abs/pii/0009898164901251Helpful?
-
-
Can the concentration be calculated using Beer's law with the extinction coefficient of cyanmethemoglobin?
1 answer-
The Molar Extinction coefficient is necessary to determine the cyanmethemoglobin concentration according to the Beer Lambert's law. The extinction coefficient has been reported in several publications. Please see the link below to review an example:
https://academic.oup.com/ajcp/article-abstract/55/2/135/1769842Using the published coefficient at a specific wavelength, Beer's law may be used to calculate the concentration of cyanmethemoglobin in a given sample, based on its absorbance at that wavelength and the path length of the cuvette. The Drabkin's Reagent will allow for the determination of the total hemoglobin concentration from a linear calibration curve and the instructions for use for this product. Please see the link below to review:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/139/718/d5941dat.pdfHelpful?
-
-
Is the hemoglobin (standard) provided suitable for use with Drabkin's Reagent (D5941)?
1 answer-
H2500 is recommended for use with Drabkin's reagent, as most mammalian hemoglobins are expected to yield similar absorbances with Drabkin reagent D5941, suggesting that porcine, rat, bovine, or human hemoglobin standards may also work. Additionally, a specific Porcine hemoglobin product, H4131, is available.
Helpful?
-
-
Can the Drabkins reagent detect hemoglobin down to 0.05 mg/dL
1 answer-
The Drabkins reagent lacks the sensitivity to detect hemoglobin at levels as low as 0.05 mg/dl, with the approximate sensitivity being in the 3 to 4 mg/dl range.
Helpful?
-
-
How to store the configured drabkin reagent and how long it can be stored
1 answer-
Store the Drabkin’s Reagent at room temperature protected from light. The powdered Drabkin’s Reagent is stable for at least 2 years. The prepared Drabkin’s Solution is stable for at least 6 months when stored at room temperature and protected from light (amber bottle).
Helpful?
-
-
what is the dilution ration that you are using for drabkin's reagent to distilled water? 1:40? Since you can't tell me what the volume is for one vial of the reagent
1 answer-
A vial of Drabkin's reagent D5941 contains 1.25 g of the powder mixture as follows:
Sodium bicarbonate, CAS # 144-55-8 || 80% (w / w) or 1000 mg per vial
Potassium ferricyanide, CAS # 13746-66-2 || 16% (w / w) or 200 mg per vial
Potassium cyanide, CAS # 151-50-8 || 4% (w / w) or 50 mg per vialThe vial should be reconstituted with 1,000 mL of water and 0.5 mL of 30 % Brij L23 Solution.
Helpful?
-
-
How many mL are in one vial of the drabkin's reagent?
1 answer-
This product is a prepared concentrate. Each vial of reagent is sufficient to prepare 1 L of Drabkin's solution. The volume of the concentrate is irrelevant, and thus not reported.
Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/139/718/d5941dat.pdfHelpful?
-
-
How much of the derbkin solution do I add for 10 mL of blood?
1 answer-
Please access the Product Datasheet below. It indicates adding 20 uL of blood to a tube containing 5 mL of the Drabkin's solution (prepared according to linked protocol). Working with a volume of blood as large as 10 mL does not seem applicable to this product:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/139/718/d5941dat.pdf
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico