TM3

91060526, mouse testis, Not specified

• UNSPSC Code: 41106514
• biological source: mouse testis
• growth mode: Adherent
• karyotype: Not specified
• morphology: Not specified
• products: Prostaglandin F2a
• receptors: Epidermal growth factor (EGF), Androgen, Oestrogen, Progesterone

Propiedades

• Nombre del producto: TM3, 91060526
• biological source: mouse testis
• growth mode: Adherent
• karyotype: Not specified
• morphology: Not specified
• products: Prostaglandin F2a
• receptors: Epidermal growth factor (EGF), Androgen, Oestrogen, Progesterone
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse BALB/c testis, Leydig cell

Cell Line Description

Originated from primary cultures of Leydig cell enriched preparations from normal testes of 11-13 day old BALB/c nu/+ mice. Characteristics similar to primary cultures of Leydig cells. Responds to leuteinising hormone but not FSH. In the presence of LH the cells are capable of metabolising cholesterol. These cells are non-tumourigenic.

Application

Hormone studies

Culture Medium

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:200 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37²C.

Other Notes

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