R2C

89031606, rat testis, Epithelial

• UNSPSC Code: 41106514
• biological source: rat testis
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
• receptors: Not specified
• relevant disease(s): cancer

Propiedades

• Nombre del producto: R2C, 89031606
• biological source: rat testis
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Rat testis Leydig cell tumour

Cell Line Description

A rat testis Leydig cell tumour cell line derived from a 2-month-old male WFu rat. Virus susceptibility: herpes simplex; vaccinia; pseudorabies; vesicular stomatitis (Indiana). Resistant to poliovirus 2. Stimulation by other hormones or cAMP is not required for hormone secretion.

Culture Medium

HAMS F10 + 2mM Glutamine + 15% Horse serum + 2.5% Foetal Bovine Serum (FBS). Do not use heat-inactivated serum.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA;5% CO₂; 37°C.

Other Notes

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