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C4018

Sigma-Aldrich

Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

lyophilized powder, ≥100 units/mg protein

Sinónimos:

3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase

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About This Item

Número de CAS:
37289-07-9
Comisión internacional de enzimas:
3.5.1.24 (BRENDA, IUBMB)
MDL number:
MFCD00130789
UNSPSC Code:
12352204
NACRES:
NA.54
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form

lyophilized powder

Quality Level

200

specific activity

≥100 units/mg protein

composition

Protein, ≥50% biuret

shipped in

wet ice

Storage temp.

−20°C

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Application

The enzyme from Sigma has been used in the analysis of bile samples in various studies.[1][2][3]

Biochem/physiol Actions

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg2+, Cu2+, and Cd2+) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.[4]

Unit Definition

One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

Physical form

Partially purified lyophilized powder containing buffer salts and stabilizer

Pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000282850
0000142044
0000188551
0000282850
0000188551

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Effects of Quercetin and Rutin on Serum and Hepatic Lipid Concentrations, Fecal Steroid Excretion and Serum Antioxidant Properties
Nakamura Y, et al.
Journal of Health Science, 46(4), 229-240 (2000)
Bile salt hydrolase activity in probiotics.
Máire Begley et al.
Applied and environmental microbiology, 72(3), 1729-1738 (2006-03-07)
Maksim Rossocha et al.
Biochemistry, 44(15), 5739-5748 (2005-04-13)
Bacterial bile salt hydrolases catalyze the degradation of conjugated bile acids in the mammalian gut. The crystal structures of conjugated bile acid hydrolase (CBAH) from Clostridium perfringens as apoenzyme and in complex with taurodeoxycholate that was hydrolyzed to the reaction
Christopher Martoni et al.
Applied microbiology and biotechnology, 81(2), 225-233 (2008-08-23)
This is the first study of its kind to screen probiotic lactic acid bacteria for the purpose of microencapsulating a highly bile salt hydrolase (BSH)-active strain. A Lactobacillus reuteri strain and a Bifidobacterium longum strain were isolated as the highest
Arekal N Roopashri et al.
Applied microbiology and biotechnology, 83(6), 1115-1126 (2009-05-02)
In a changing scenario of food habits being associated with wellness factors through the concepts of probiotics and prebiotics, an attempt has been made to characterize on molecular basis, the desirable benefits associated with natural isolates of lactic acid bacteria
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