C0992

Monoclonal Anti-Cy3/Cy5 antibody produced in mouse

clone CY-96, purified from hybridoma cell culture

• Sinónimos: Monoclonal Anti-Cy3/Cy5, Cy3 Antibody, Cy3 Antibody - Anti-Cy3/Cy5 antibody, Mouse monoclonal
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: CY-96, monoclonal
• Formulario: buffered aqueous solution
• concentración: ~1.5 mg/mL
• técnicas: direct ELISA: suitable; dot blot: 1-2 μg/mL using cell protein extracts labeded with Cy3 or Cy5; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable
• isotipo: IgG2a
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Anti-Cy3/Cy5 antibody, Mouse monoclonal, (mouse IgG2a isotype) is derived from the hybridoma CY-96 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from Balb/c mice immunized with a mixture of proteins labeled with Cy3 or Cy5.

Especificidad

The antibody recognizes Cy3 and Cy5 conjugated to proteins.

Inmunógeno

mixture of proteins labeled with Cy3/Cy5.

Aplicación

Anti-Cy3/Cy5 antibody, Mouse monoclonal has been used in:

• immunofluorescence
• western blot
• dot blot
• enzyme linked immunosorbent assay (ELISA)
• immunoprecipitation
• immunocytochemistry
• protein microarrays
• In in situ hybridization

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Acciones bioquímicas o fisiológicas

Cy 3 and Cy5 are the most popular cyanine dyes, used combined for two color detection. Cy3 dyes are fluorescent orange while Cy5 is fluorescent in the red region. Cyanine belonging to polymethine group. CyDyes are a family of fluorophores that can be used for labeling proteins, peptides, DNA, RNA, and other biomolecules. These dyes are small, pH insensitive, soluble in aqueous solution and are tolerant to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. Mainly Cy3 and Cy5 are used in many different biological assays such as DNA microarrays, protein microarrays, two-dimensional protein analysis (2D gels), fluorescence resonance energy transfer (FRET), and immunocytochemistry.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)