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BCK-EDU555

Sigma-Aldrich

ClickTech EdU Cell Proliferation Kit 555 for IM

sufficient for 100 assays

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.22

fluorescencia

λex 546 nm; λem 579 nm

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Descripción general

EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the Baseclick EdU-Click Assays utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.

Aplicación

Imaging

Pictogramas

Health hazardExclamation mark
GHS08,GHS07

Palabra de señalización

Danger

Frases de peligro

H319,H340,H361,H412

Clasificaciones de peligro

Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 1B - Repr. 2

Código de clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a

Contenido relacionado

Baseclick EdU Cell Proliferation Detection Kits: Improve Data Quality and Save Time

Experimental studies measuring cell proliferation have had implications in cancer biology, immunology, cell biology, and developmental biology.

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