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Merck

B9146

Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

≥98%, powder, suitable for identification of lac+ bacterial colonies

Sinónimos:

5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

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About This Item

Fórmula empírica (notación de Hill):
C14H15BrClNO6
Número de CAS:
Peso molecular:
408.63
Beilstein:
1402009
Número CE:
Número MDL:
Código UNSPSC:
12352204
ID de la sustancia en PubChem:
NACRES:
NA.83

Nombre del producto

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, ≥98%

Nivel de calidad

Ensayo

≥98%

Formulario

powder

solubilidad

DMF: methanol (1:1): soluble 50 mg/mL

idoneidad

suitable for identification of lac+ bacterial colonies

temp. de almacenamiento

−20°C

cadena SMILES

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

Clave InChI

OPIFSICVWOWJMJ-AEOCFKNESA-N

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Aplicación

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used to confirm transduction of Caki-2/LacZ clones. It has also been used in blue-white selection of transformants.
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Reconstitución

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20 °C and protected from light. Sterilization is not required.

Producto relacionado

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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