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AB0100

Sigma-Aldrich

Alkaline Phosphatase Blue Microwell Substrate

alkaline phosphatase substrate, solution

Sinónimos:

5-bromo-4-chloro-3-indolyl phosphate analog / NBT substrate for microwell assays

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

Nombre del producto

Alkaline Phosphatase Blue Microwell Substrate, sufficient for 400 mL working substrate

form

solution

Quality Level

200

usage

sufficient for 400 mL working substrate

storage temp.

2-8°C

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General description

Alkaline phosphatase blue microwell substrate, a two-component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require an insoluble reaction product.

Application

Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue solution, and the Nitro BT reagent is a yellow solution. The two component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require a precipitation reaction product.

Physical form

A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a 2 component buffered alkaline phosphatase substrate containing Bromo-Chloro-Indolylphosphate (BCIP) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

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Certificados de análisis (COA)

Lot/Batch Number
SLCP3050
SLCM1719
SLCN5760
SLCP2519
SLCM7961

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Long noncoding RNAs (lncRNAs) have been implicated in diverse biological processes, including embryonic stem cell (ESC) maintenance. However, their functional mechanisms remain largely undefined. Here, we show that the lncRNA Panct1 regulates the transient recruitment of a putative X-chromosome-encoded protein
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Stem cells (Dayton, Ohio), 40(1), 35-48 (2022-05-06)
DNA damage repair (DDR) is a safeguard for genome integrity maintenance. Increasing DDR efficiency could increase the yield of induced pluripotent stem cells (iPSC) upon reprogramming from somatic cells. The epigenetic mechanisms governing DDR during iPSC reprogramming are not completely
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Moyer, et al.
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Artículos

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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