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Merck

A8311

Sigma-Aldrich

Antiespumante 204

aqueous emulsion for bacterial and mammalian systems

Sinónimos:

organic antifoam

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About This Item

MDL number:
UNSPSC Code:
12161901
NACRES:
NA.85

Quality Level

form

emulsion (aqueous)

concentration

100% (organic)

density

1.01 g/mL at 25 °C

application(s)

microbiology

storage temp.

room temp

suitability

(Mammalian (suspension))
bacteria (fermentation)

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General description

Antifoam 204 is an organic antifoam used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Application

Suitable for preventing or minimizing foaming in microbial fermentation systems and mammalian suspension cultures.
Antifoam 204 has been used:
  • in the expression and purification of PUL (PLAP, Ufd3p, and Lub1p) domain
  • in fed-batch fermentation
  • to prevent bubble formation due in aeration in Luria Bertani (LB) culture medium for bacterial growth
  • in the batch fermentation with mineral medium

Components

Antifoam 204 is a 100% active components and a mixture of non-silicone organic defoamers in a polyol dispersion.

Other Notes

Contiene componentes activos al 100 % y es una mezcla de antiespumantes orgánicos distintos de la silicona en una dispersión poliol. Puede esterilizarse repetidamente.

Preparation Note

For use in microbiological media Sigma recommends a starting concentration of between 0.005% and 0.01%.
The optimal amount of antifoam required for various applications will need to be determined. Antifoam 204 is soluble in methanol, ethanol, toluene, xylene, perchloroethylene, and cold water at temperatures below 15 °C. It is insoluble in warm water and ethylene glycol.

related product

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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