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A6017

Sigma-Aldrich

ω-Aminohexyl–Agarose

saline suspension

Sinónimos:

Omega-Aminohexyl-Agarose, 1,6-Diaminohexane–Agarose (N-linked), 6-Aminohexyl–Agarose

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About This Item

Número de CAS:
58856-73-8
MDL number:
MFCD00801286
UNSPSC Code:
23151817
NACRES:
NA.56

biological source

plant

Quality Level

200

form

saline suspension

extent of labeling

≥5 μmol per mL

technique(s)

affinity chromatography: suitable

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

amino of 1,6-diaminohexane

matrix spacer

1 atom

capacity

≥5 mg/mL binding capacity (bovine serum albumin)

suitability

suitable for chromatography

storage temp.

2-8°C

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General description

Hydrophobic ligands can serve as potentselective adsorbents. Both ω-aminoalkyl and alkyl agaroses can interact with the hydrophobic regions found in various proteins. The ω-aminoalkyl agarose product offered here consists of activated agarose with 1,6-diaminohexane covalently attached to one of its amine groups.

Application

ω-Aminohexyl–Agarose has been used in the conjugation of fatty acids with chain length from two carbons to 20 for fatty acyl beads pull-down experiments.
ω-aminohexyl–agarose has been used for coupling of serotype b-specific carbohydrate antigen (SbAg) of Actinobacillus actinomycetemcomitans Y4 for isolation of anti-SbAg antibodies using protein chromatography.

Biochem/physiol Actions

ω-aminoalkyl-agaroses are used in chromatography. In these, the protein is retained by lipophilic association between the hydrocarbon side chains on the agarose and hydrophobic pockets in the protein.

Physical form

Suspension in 0.5 M NaCl containing preservative

Other Notes

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H315,H318

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000362926
0000362927
0000348305
0000280011
0000362926

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L G Martini et al.
Pharmaceutical research, 12(11), 1786-1790 (1995-11-01)
The purpose of this study was to investigate the influence of hydration characteristics on the in vitro release of 5-fluorouracil from a swellable matrix prepared using a novel triblock copolymer of poly(epsilon-caprolactone) and poly(oxyethylene). Matrices were prepared by dry compression
S Mandjiny et al.
Journal of chromatography, 616(2), 189-195 (1993-07-02)
Histidine, a pseudobiospecific ligand, had been utilized to purify several proteins such as chymosin, acidic protease, carboxypeptidase Y and immunoglobulin G (IgG). A detailed study was undertaken to purify IgG on histidine coupled to aminohexyl Sepharose [A. El-Kak and M.
Heterogeneity of antibodies reactive with the dominant antigen of Actinobacillus actinomycetemcomitans.
Nakashima K, et al.
Infection and Immunity, 65, 3794-3798 (1997)
Use of stable 6-aminohexyl derivatives for labelling polysaccharides with haptens and for preparing polysaccharide immunoadsorbents.
P L Ey
Journal of immunological methods, 160(1), 135-137 (1993-03-15)
D Couchie et al.
The Biochemical journal, 199(2), 441-446 (1981-11-01)
Chromatography on hexyl-agarose resolved a partially purified cyclic GMP-activated phosphodiesterase from rat liver into two peaks of activity: the first was eluted with 0.5 M-KCl and was cyclic AMP-specific. The second was tightly bound to hexyl-agarose and was not eluted
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