A2230
Apirasa from potatoes
High Activity, ATPase ≥600 units/mg protein, lyophilized powder
Sinónimos:
Adenosina 5′-difosfatasa, Adenosina 5′-trifosfatasa
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About This Item
Número de CAS:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Productos recomendados
biological source
potato
Quality Level
form
lyophilized powder
quality
High Activity
ATPase activity
≥600 units/mg protein
secondary activity
≥50 % of base activity ADPase
composition
protein, ≥30%
foreign activity
Acid Phosphatase ≤2% of base activity
shipped in
wet ice
storage temp.
−20°C
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Application
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation . Product A2230 is a high activity apyrase.
Se encuentran al menos dos isoenzimas en diferentes variedades de S. tuberosum: una con una elevada relación ATPasa/ADPasa (∼10) y otra con una relación baja (∼1).
Reacción: ATP → ADP+Pi → AMP+2Pi.
Reacción: ATP → ADP+Pi → AMP+2Pi.
Biochem/physiol Actions
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Packaging
Sold on the basis of ATPase units.
Unit Definition
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
Physical form
Lyophilized powder containing potassium succinate buffer salts.
Preparation Note
Derived from red potato
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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