Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

Ver Toda la documentación

73737

Sigma-Aldrich

4-Nitrophenyl phosphate bis(tris) salt

phosphatase substrate, ≥97.0% (enzymatic), powder or crystals

Sinónimos:

p-Nitrophenylphosphate bis(tris) salt, p-nitrophenyl phosphate bis(tris) salt, PNPP, PNPP bis(tris) salt

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Fórmula lineal:
(C6 H4 N O6 P)( C4 H12 N O3)2
Número de CAS:
68189-42-4
Peso molecular:
461.36
Beilstein/REAXYS Number:
1578142
EC Number:
269-198-6
MDL number:
MFCD00037009
UNSPSC Code:
12352204
PubChem Substance ID:
57652488
NACRES:
NA.32

Nombre del producto

4-Nitrophenyl phosphate bis(tris) salt, for the determination of phosphatase, ≥97.0% (enzymatic)

Quality Level

100

assay

≥97.0% (enzymatic)

form

powder or crystals

quality

for the determination of phosphatase

impurities

≤0.1% free nitrophenol
≤6% water

solubility

H2O: 0.1 g/mL, clear

storage temp.

2-8°C

SMILES string

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

¿Está buscando productos similares? Visita Guía de comparación de productos

Application

4-Nitrophenyl phosphate bis(tris) salt has been used to determine the enzyme activity of acid and alkaline phosphatase in cell lysates.

Biochem/physiol Actions

4-Nitrophenyl phosphate is a non-proteinaceous and non-specific chromogenic substrate, preferred for alkaline phosphatase (ALP) in enzyme-linked immunosorbent assay (ELISA). It is widely used to assay protein tyrosine phosphatases (PTPs). Upon cleavage, the substrate produces a yellow product that is spectrophotometrically measured.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
BCCD6826
BCCC2771
BCBW6778
BCBQ1676V
BCBN3446V

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

D.W. Moss
Methods of Enzymatic Analysis, 92-92 null
Elif Özlem Arslan-Aydoğdu et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 49(1), 189-199 (2017-10-19)
Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospholipase
Ulrike Lorenz
Current protocols in immunology, Chapter 11, Unit 11-Unit 11 (2011-04-05)
Tyrosine phosphorylation and dephosphorylation of proteins play a critical role in many processes of the immune system, from early development to fully differentiated effector function. Since the opposing actions of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs) determine
Mary E Wood et al.
Vaccine, 36(50), 7737-7743 (2018-11-12)
Chronic wasting disease (CWD) is a fatal prion disease affecting multiple cervid species. Effective management tools for this disease, particularly in free-ranging populations, are currently limited. We evaluated a novel CWD vaccine in elk (Cervus canadensis) naturally exposed to CWD
Enzyme-linked Immunosorbent Assay (ELISA): From A to Z null
Ver todos los artículos relacionados

Artículos

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico