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Sigma-Aldrich

Atto 550-Biotin

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

Código UNSPSC:
12352108
eCl@ss:
34058011
NACRES:
NA.32

Línea del producto

BioReagent

Nivel de calidad

100

Ensayo

≥90% (HPLC)

Formulario

film

fabricante / nombre comercial

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

Absorción UV

λ: 553-559 nm Amax

idoneidad

suitable for fluorescence

temp. de almacenamiento

−20°C

Aplicación

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies. Atto 550-Biotin is suitable for fluorescence labeling of avidin/streptavidin conjugated molecules.

Información legal

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
BCCM6555
BCCL9503
BCCL7590
BCCK9301
BCCH5895

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Atto 550

Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
J Oertel et al.
Journal of cancer research and clinical oncology, 126(8), 425-440 (2000-08-29)
Immunocytochemical investigations of fixed cells are used to enhance diagnostic accuracy in haematology and oncology. The alkaline-phosphatase/anti(alkaline phosphatase) technique, immunoperoxidase and the avidin-biotin technique are the most important methods in immunocytochemistry. Tyramide-enhanced immunostaining is a new powerful technique. Peripheral blood
Immunohistochemical methods for the demonstration of tumor markers.
H Denk
Current topics in pathology. Ergebnisse der Pathologie, 77, 47-69 (1987-01-01)
J Chevalier et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45(4), 481-491 (1997-04-01)
Biotin was recently applied to detect cellular DNA or RNA. In combination with avidin, streptavidin or antibody, it can be conjugated with fluorescent dye, enzyme, ferritin, or gold. However, emphasis has recently been placed on the false-positive results that are
A molecular toolkit for population genetic investigations of the ash dieback pathogen Hymenoscyphus pseudoalbidus.
Gross, A., et al.
Forest Pathology, 42, 252-264 (2012)
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