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Merck

861340

Sigma-Aldrich

Thionin acetate salt

certified by the Biological Stain Commission, Dye content ≥85 %

Sinónimos:

3,7-Diamino-5-phenothiazinium acetate, Lauth’s violet

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About This Item

Fórmula empírica (notación de Hill):
C12H9N3S · C2H4O2
Número de CAS:
Peso molecular:
287.34
Colour Index Number:
52000
Beilstein/REAXYS Number:
4345073
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, ≥85%

technique(s)

microbe id | staining: suitable

λmax

598 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CC([O-])=O.Nc1ccc2nc3ccc(N)cc3[s+]c2c1

InChI

1S/C12H10N3S.C2H4O2/c13-7-1-3-9-11(5-7)16-12-6-8(14)2-4-10(12)15-9;1-2(3)4/h1-6H,13-14H2;1H3,(H,3,4)/q+1;/p-1

InChI key

OWXBIRAFHWASMS-UHFFFAOYSA-M

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General description

Thionin is a diaminothiazine, metachromatic cationic dye.

Application

Thionin is widely used as a biological stain, especially in histology.[1] It has been used in the Nissl stain for the labeling of neuronal cell bodies.[2]

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Questions

  1. 製品No.861340(チオニン 酢酸塩)の内容量は ≥85%となっていますが、残りはチオニン酢酸塩由来の不純物ということでしょうか?それともそれ以外のものになりますでしょうか? また、こちらの製品の水への溶解度を教えていただくことは可能でしょうか? 宜しくお願い致します。

    1 answer
    1. There is no testing for either dye purity of dye impurities for product 861340, Thionin Acetate. The dye content is calculated using the spectral characteristics testing procedure from the Biological Stain Commission. The method can be found in the following publication:

      Penney, D. P., Powers, J. M., Frank, M., Willis, C., & Churukian, C. (2002). Analysis and testing of biological stains-- The Biological Stain Commission Procedures. Biotechnic & Histochemistry, 77(5–6), 237–275. https://doi.org/10.1080/bih.77.5-6.237.275.

      There is no testing for the impurities that are present.

      As to the solubility in water, the literature is devoted exclusively to the chloride salt. The Aldrich Handbook of Stains, Dyes and Indicators by Floyd Green states the literature is devoted almost exclusively to the chloride salt. There is mention of 30 mg/ml solubility, but it is somewhat unclear which form of Thionin is being mentioned. There is also a statement that the Thionin Acetate "is much more soluble in water and the short chain alcohols than the chloride."

      The exact solubility in water is unknown, but should be greater than 30 mg/ml. A typical concentration for Thionin for Nissl Substance is 0.5%. The Clark method for Nissl substance (1945) uses a concentration of 25 mg/ml or 0.25%. Thionin can be used for a variety of techniques. The dye is also used as a nuclear stain at 0.5% in 20% alcohol. The expectation is that the conditions and concentrations will vary depending upon the test method.

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