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Merck

68724

Supelco

Fast Green FCF

analytical standard

Sinónimos:

Food green 3

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About This Item

Fórmula lineal:
C37H34N2O10S3Na2
Número de CAS:
Peso molecular:
808.85
Colour Index Number:
42053
Beilstein/REAXYS Number:
5718212
EC Number:
MDL number:
UNSPSC Code:
85151701
PubChem Substance ID:
E Number:
E143
NACRES:
NA.24

grade

analytical standard

Quality Level

assay

≥98.0% (HPLC)

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

mp

290 °C (dec.) (lit.)

application(s)

cleaning products
cosmetics
food and beverages
personal care

format

neat

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O

InChI

1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2

InChI key

RZSYLLSAWYUBPE-UHFFFAOYSA-L

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General description

Fast Green FCF is a synthetic organic dye, which belongs to the triphenylmethane group. It can be found in a wide range of applications in food and beverage industry. It can also be used in scientific research for the staining of proteins and identification of extracellular recording or injection sites in electrophysiological experiments.

Application

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

Health hazardExclamation mark

signalword

Warning

hcodes

Hazard Classifications

Muta. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

352.4 °F - closed cup

flash_point_c

178 °C - closed cup


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Los clientes también vieron

Fast Green FCF (Food Green 3) inhibits synaptic activity in rat hippocampal interneurons
Hooft V.AJ
Neuroscience Letters, 318, 163-165 (2002)
Nonlinear responses and optical limiting behavior of fast green FCF dye under a low power CW He?Ne laser irradiation
Jamshidi-Ghaleh K, et al.
Optics Communications, 271, 551-554 (2007)
L B Gilmore et al.
Journal of biochemical and biophysical methods, 5(1), 57-66 (1981-07-01)
The quantitation of proteins in polyacrylamide gels stained with Fast green FCF has been investigated using a modification of the elution technique originally described by Fenner et al. (Fenner, C., Traut, R.R., Mason, D.T. and Wikman-Coffelt, J. (1975) Anal. Biochem.
Michael J Ford et al.
Rapid communications in mass spectrometry : RCM, 18(12), 1303-1309 (2004-06-03)
A combined surface sampling probe/electrospray emitter coupled with an ion trap mass spectrometer was used for the direct read out of unmodified reversed-phase C18 thin-layer chromatography (TLC) plates. The operation of the surface sampling electrospray ionization interface in positive and
A C Dhar et al.
Stain technology, 57(3), 151-155 (1982-05-01)
Fast green FCF was used to localize acidic nuclear proteins in sections of young flower buds of Limnophyton obtusifolium (L.) Miq. After extracting nucleic acids, the slides were stained at hydrogen ion concentrations ranging from pH 2.6 to 9.0. At

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