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114413

Sigma-Aldrich

Bromothymol Blue

ACS reagent, Dye content 95 %

Sinónimos:

3′,3″-Dibromothymolsulfonphthalein

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About This Item

Fórmula empírica (notación de Hill):
C27H28Br2O5S
Número de CAS:
76-59-5
Peso molecular:
624.38
Beilstein/REAXYS Number:
373934
EC Number:
200-971-2
MDL number:
MFCD00005872
UNSPSC Code:
12171500
PubChem Substance ID:
24847171
NACRES:
NA.47

grade

ACS reagent

Quality Level

200

form

powder

composition

Dye content, 95%

color

pink-purple

visual transition interval

6.0-7.6, yellow to blue(passes test)

mp

200-202 °C ((392 - 396 °F) - lit.)
200-202 °C (lit.)

λmax

420 nm

ε (extinction coefficient)

≥17000 at 419-425 nm in methanol
≥8000 at 326-332 nm in methanol
≥9000 at 279-285 nm in methanol

storage temp.

room temp

SMILES string

CC(C)c1cc(c(C)c(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)c(Br)c4C

InChI

1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3

InChI key

NUHCTOLBWMJMLX-UHFFFAOYSA-N

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General description

Bromothymol Blue, also known as, dibromothymolsulfonphthalein is a sulfonated hydroxyquinone in acidic aqueous solutions. In alkaline solutions, this dye is present as a moderately sized dianion. It is also known as bromthymol blue and dibromothymolsulfonphthalein. It is a pH-sensitive dye that changes color from yellow to blue.

Application

Bromothymol blue dye serves as a pH indicator because of the precise color transition at neutrality. Other applications include:
  • as a vital stain to trace the movement of fluids from the lymph and to define cell walls or nuclei under the microscope
  • for the demonstration of fungal hyphae within plant roots
  • in the assessment of the soluble acidity within single rice grains
  • use in an agar gel medium for enumeration of Bacillus cereus
  • as a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000352443
0000352443
SHBQ9942
0000293123
0000234446

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Andrea Pastore et al.
Talanta, 219, 121251-121251 (2020-09-06)
A pH colorimetric sensor array (CSA) with fast response time (<1 min) using only one acid-base indicator, Bromothymol Blue (BB), was prepared and characterized by modulating the amount, C, of the surfactant Hexadecyltrimethylammonium p-toluenesulfonate between 0 and 0.3725 gCTApTs/gprecursor with a
Reza Amin et al.
Micromachines, 11(6) (2020-07-01)
To transform from reactive to proactive healthcare, there is an increasing need for low-cost and portable assays to continuously perform health measurements. The paper-based analytical devices could be a potential fit for this need. To miniaturize the multiplex paper-based microfluidic
Hisataka Maruyama et al.
Lab on a chip, 8(2), 346-351 (2008-01-31)
This paper demonstrates local pH measurement in a microchip using a pH-sensing gel-microbead. To achieve this, the gel-microbead made of a hydrophilic photo-crosslinkable resin was functionalized with the pH indicator bromothymol blue (BTB). The primary constituent of this photo-crosslinkable resin
Mariana A Sanchez et al.
Analytica chimica acta, 694(1-2), 95-99 (2011-05-14)
Liquid-liquid microextraction without phase segmentation was implemented in a multicommuted flow system for determination of the anti-hypertensive diltiazem. The procedure was based on ion pair formation between the drug and the dye bromothymol blue at pH 3.5. The detection was
Kristina Jurcic et al.
Journal of chromatography. A, 1134(1-2), 317-325 (2006-10-07)
An effective sample preconcentration technique for proteins and peptides was recently developed using capillary electrophoresis (CE) with discontinuous buffers [C.A. Nesbitt, J.T.-M. Lo, K.K.-C. Yeung, J. Chromatogr. A 1073 (2005) 175]. Two buffers of different pH created a junction to
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