This product is a proprietary formulation that utilizes a mix of mild detergents and protein stabilization buffer that, when combined with tris(hydroxypropyl)phosphine (THP)
reducing agent, resulting in higher total protein yields the recovery of enzymatically active proteins.
71186
YeastBuster Protein Extraction Reagent
Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis
Sinónimos:
Yeast protein extraction reagent
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About This Item
Código UNSPSC:
41106512
NACRES:
NA.77
En este momento no podemos mostrarle ni los precios ni la disponibilidad
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Formulario
liquid
fabricante / nombre comercial
Novagen®
condiciones de almacenamiento
OK to freeze
Condiciones de envío
wet ice
Descripción general
Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis
YeastBuster<TMSYMBOL></TMSYMBOL> Protein Extraction Reagent is formulated for a fast, efficient and gentle extraction of active proteins from yeast cells. The reagent avoids harsh conditions of vigorous mechanical or chemical treatment that often result in heat degradation of target proteins. The proprietary formulation utilizes a mix of mild detergent, protein stabilization buffer, and tris(hydroxypropyl) phosphine (THP) reducing agent (THP concentrate provided separately). This powerful combination eliminates the inconsistencies associated with tedious mechanical disruption of yeast cells with glass bead abrasives, ultrasonication and pressure disruption, or enzymatic digestion with β-1,3-glucanase lytic enzymes. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, and Schizosaccharomyces pombe strains and with plant cells.
In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind<TMSYMBOL></TMSYMBOL> and Ni-NTA HIS-BIND® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.
In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind<TMSYMBOL></TMSYMBOL> and Ni-NTA HIS-BIND® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.
Características y beneficios
- Gentle, rapid, efficient extraction of proteins from yeast cells
- Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
- Higher yield of total and enzymatically active proteins
- Fully compatible with Ni-NTA His•Bind and GST•Bind affinity purification methods
Componentes
•100 ml or 500 mlYeastBuster Protein Extraction Reagent
•1 ml or 5 ml100X THP Solution
•1 ml or 5 ml100X THP Solution
Advertencia
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Otras notas
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Información legal
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
STOT RE 2 Oral
Órganos de actuación
Kidney
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast
Contenido relacionado
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Los métodos de lisis celular y extracción de proteínas abarcan varias técnicas, desde la solubilización de detergentes hasta la ruptura mecánica, que respaldan las necesidades de investigación.
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Does YeastBuster denature the enzymes that are liberated from yeast? Or generally do most intracellular enzymes retain activity? Thanks.
1 answer-
Helpful?
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Cat #71186 YeastBuster Protein Extraction Reagent inquiry: What are the ingredients of this reagent? Is there a detergent in the reagent? I understand if the actual concentrations are proprietary, but I would appreciate knowing what the ingredients are.
1 answer-
As provided, this proprietary formulation containing ethylene glycol, lithium chloride and Tris(hydroxypropyl)phosphine is in a mixture with mild zwitterionic detergent.
Helpful?
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