The Sigma-Aldrich Anti-α-Actin Mouse mAb (1A4) with the product number 113200 is the same "clone" as the Sigma-Aldrich Anti-Actin, smooth muscle Antibody, clone ASM-1/1A4 with the product number CBL171-I. Clone ASM-1, also known as anti-αsm-1 and clone 1A4. The Anti-Actin smooth muscle Antibody, clone ASM-1/1A4 (product number CBL171-I) shows reactivity in human, mouse, rat, chicken, and bovine species. Accordingly, the Anti-α-Actin Mouse mAb (1A4) with the product number 113200 should cross-react with bovine smooth muscle actin since it is the same clone as in CBL171-I.
113200
Anti-α-Actin Mouse mAb (1A4)
liquid, clone 1A4, Calbiochem®
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.41
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
origen biológico
mouse
Nivel de calidad
tipo de anticuerpo
primary antibodies
clon
1A4, monoclonal
Formulario
liquid
contiene
≤0.1% sodium azide as preservative
reactividad de especies
baboon, rabbit, human
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
do not freeze
isotipo
IgG2a
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... ACTA1(58)
Descripción general
Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Antibody Target Gene Symbol: ACTA2 Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN Entrez Gene Name: actin, alpha 2, smooth muscle, aorta Hu Entrez ID: 59 (Related Antibodies: 113200) Rat Entrez ID: 81633
This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.
Inmunógeno
Human Smooth Muscle
a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
Aplicación
Frozen Sections (1:50-1:75)
Immunoblotting (see comments)
Paraffin Sections (1:50-1:75)
Immunoblotting (see comments)
Paraffin Sections (1:50-1:75)
Envase
Please refer to vial label for lot-specific concentration.
Advertencia
Toxicity: Standard Handling (A)
Forma física
In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
Nota de análisis
Negative Control
Fibroblasts, striated muscle, or myocardium
Fibroblasts, striated muscle, or myocardium
Positive Control
Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
Otras notas
Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Could you provide information on the reactivity of antibody 113200-500UL with bovine cells? The website does not mention reactivity for bovine species, and I would like to use it with bovine cells.
1 answer-
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