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Merck

198080

Sigma-Aldrich

Methyl Green

zinc chloride salt, certified by the Biological Stain Commission, Dye content 85 %

Sinónimos:

Ethyl Green

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About This Item

Fórmula lineal:
C27H35BrClN3 · ZnCl2
Número de CAS:
Peso molecular:
653.24
Colour Index Number:
42590
Beilstein/REAXYS Number:
3914106
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

powder

composition

Dye content, 85%

technique(s)

titration: suitable

pH

0.2-1.8, yellow to blue

mp

>300 °C (lit.)

solubility

H2O: 1 mg/mL

λmax

423 nm (2nd)
629 nm

ε (extinction coefficient)

≥11100 at 417-425 in H2O at 0.005 g/L
≥16000 at 311-319 in H2O at 0.005 g/L
≥60000 at 626-634 in H2O at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C

InChI

1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4

InChI key

RRKSQWOQZFIQKW-UHFFFAOYSA-J

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General description

Methyl Green is a basic dicationic dye and is composed of triphenylmethane. It is suitable for the detection of DNA and RNA. It acts as a photochromophore and used to activate gelatinous films. At a very high pH, methyl green forms an alkaline solution and gets converted to a colorless carbinol base. In the presence of hydroxide anion, methyl green produces crystal violet dye and ethanol.

Suitability

Suitable for use as a DNA stain.

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

wgk_germany

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Certificados de análisis (COA)

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Visite la Librería de documentos

Mechanistic studies of the photocatalytic degradation of methyl green: an investigation of products of the decomposition processes
Chen CC and Lu CS
Environmental Science & Technology, 41(12), 4389-4396 (2007)
R.D. Lillie Ed.
Conn?s Biological Stains, 282-282 (1977)
Virchow?s Arch. (Pathol. Anat.), 157, 19-19 (1899)
The problem of a standardized technic for the methyl-green-pyronin stain.
E B TAFT
Stain technology, 26(3), 205-212 (1951-07-01)
Bethany Dado-Senn et al.
PloS one, 14(9), e0222120-e0222120 (2019-09-20)
The bovine dry period is a non-lactating period between consecutive lactations characterized by mammary gland involution and redevelopment phases to replace senescent mammary epithelial cells with active cells primed for the next lactation. Dairy cows exposed to heat stress during

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