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SAB4200394

Sigma-Aldrich

Anti-Nestin (C-terminal) antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody

Synonym(s):

Anti-NES

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~300 kDa

species reactivity

mouse, rat

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

technique(s)

indirect immunofluorescence: 1-2 μg/mL using mouse P19 cells
western blot: 2-4 μg/mL using lysates of HEK293T cells over-expressing rat nestin.

UniProt accession no.

P21263

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Nes(18008)
rat ... Nes(25491)

General description

Nestin/NES belongs to the intermediate filament (IF) protein family. Its expression is found in immature neuroepithelial and progenitor cell types. This 240 kDa protein contains the shortest head domain (N-terminus) and a long tail domain (C-terminus). Nestin is mapped to human chromosome 1q23.1.

Immunogen

synthetic peptide corresponding to a sequence at the C- terminus of mouse nestin, conjugated to KLH. The sequence is identical in rat nestin.

Application

Anti-Nestin (C-terminal) antibody has been used in immunostaining.

Biochem/physiol Actions

Nestin/NES plays a major role in the modulation of assembly and disassembly of intermediate filaments, which in turn help in the remodeling of the cell along with other structural proteins.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nestin structure and predicted function in cellular cytoskeletal organisation.
Michalczyk K and Ziman M
Histology and Histopathology, 20(2), 665-671 (2005)
Intermediate filaments in peripheral nervous system: Their expression, dysfunction and diseases
Parlakian A, et al.
Revista de Neurologia, 172(10), 607-613 (2016)
Nestin expression in embryonic human neuroepithelium and in human neuroepithelial tumor cells.
Tohyama T, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 66(3), 303-313 (1992)
The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons.
Zhu B, et al.
Biochemical and Biophysical Research Communications, 500(3), 645-649 (2018)
YAP stabilizes SMAD1 and promotes BMP2-induced neocortical astrocytic differentiation.
Huang Z, et al.
Development, 143(13), 2398-2409 (2016)
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