C-39625

Follicle Dermal Papilla Cell Growth Medium

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

• Synonym(s): Recommended with C-26500
• UNSPSC Code: 12352207
• NACRES: NA.81

Properties

• form: aqueous solution
• packaging: pkg of 1 vial
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Follicle Dermal Papilla Cell Growth Medium SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-26500

Application

PromoCell Follicle Dermal Papilla Cell Growth Medium is a low-serum medium that has been developed for the in vitro cultivation of Human Follicle Dermal Papilla Cells. The medium is optimized for primary cultures of fetal, neonatal, and adult tissue, or for the maintenance of follicle dermal papilla cells.Recommended for:Human Follicle Dermal Papilla Cells

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Quality

All lots of PromoCell Follicle Dermal Papilla Cell Growth Medium are subjected to comprehensive quality control tests using primary human follicledermal papilla cells. Each lot is checked for growth promoting activity, adherence rate and typical morphology of the tested follicle dermal papilla cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.04 ml / mlBovine Pituitary Extract: 0.004 ml / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml

Storage and Stability

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

Click here for more information.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable